Investigating the probiotic characteristics of four microbial strains with potential application in feed industry

Jung-Ae Kim; Joel Bayo; Juncheol Cha; Yeon Jae Choi; Min Young Jung; Dae-Hyuk Kim; Yangseon Kim

doi:10.1371/journal.pone.0218922

Investigating the probiotic characteristics of four microbial strains with potential application in feed industry

PLoS One. 2019 Jun 26;14(6):e0218922. doi: 10.1371/journal.pone.0218922. eCollection 2019.

Authors

Jung-Ae Kim¹, Joel Bayo², Juncheol Cha³, Yeon Jae Choi⁴, Min Young Jung¹, Dae-Hyuk Kim^{1

5}, Yangseon Kim¹

Affiliations

¹ Center for Industrialization of Agricultural and Livestock Microorganisms, Jeongeup-si, Korea.
² Department of Agricultural Convergence Technology, Chonbuk National University, Jeonju-si, Korea.
³ Chemport Inc. Naju-si, Korea.
⁴ International Agricultural Development and Cooperation Center, Chonbuk National University, Jeonju-si, Korea.
⁵ Department of Molecular Biology, Chonbuk National University, Jeonju-si, Korea.

Abstract

The present study aimed to evaluate the probiotic characteristics of certain microbial strains for potential use as feed additives. Three bacterial strains and a yeast previously isolated from different environments were investigated. The strains were subjected to molecular identification and established as Lactobacillus paracasei CP133, Lactobacillus plantarum CP134, Bacillus subtilis CP350 and Saccharomyces cerevisiae CP605. Lactobacillus sp. CP133 and CP134 exhibited antibiosis, antibiotic activity, and relative odor reduction ability. Bacillus subtilis CP350 was thermotolerant, reduced hydrogen sulfide gas and showed significant proteolytic activity, whereas Saccharomyces cerevisiae CP605 exhibited high acid and bile salt tolerance. In general, the isolates in this study demonstrated improved functional characteristics, particularly acid and bile tolerance and relative cell adhesion to HT-29 monolayer cell line. Results in this work provides multifunctional probiotic characteristics of the strains for potential development of probiotics and cleaning of the environment.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animal Feed / microbiology*
Anti-Bacterial Agents / pharmacology
Antibiosis / drug effects
Bacillus subtilis / growth & development
Bacteria / growth & development*
Bile Acids and Salts / pharmacology
Cell Adhesion / drug effects
Cell Line, Tumor
Food Microbiology / methods
HT29 Cells
Humans
Lacticaseibacillus paracasei / growth & development
Lactobacillus plantarum / growth & development
Probiotics / pharmacology*
Staphylococcus aureus / growth & development

Substances

Anti-Bacterial Agents
Bile Acids and Salts

Grants and funding

This research was supported by the Next-Generation BioGreen 21 Program (Project No. PJ01322304), Rural Development Administration, Republic of Korea to YK and the Strategic Initiative for Microbiomes in Agriculture and Food grant no. 918002-4, Ministry of Agriculture, Food and Rural Affairs, Republic of Korea to YK. Chemport Inc. provided support in the form of salaries for author J. Cha, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of this author are articulated in the ‘author contributions’ section.