Detection of plasmid-mediated tigecycline-resistant gene tet(X4) in Escherichia coli from pork, Sichuan and Shandong Provinces, China, February 2019

Li Bai; Pengcheng Du; Yinju Du; Honghu Sun; Pei Zhang; Yuping Wan; Qi Lin; Séamus Fanning; Shenghui Cui; Yongning Wu

doi:10.2807/1560-7917.ES.2019.24.25.1900340

Detection of plasmid-mediated tigecycline-resistant gene tet(X4) in Escherichia coli from pork, Sichuan and Shandong Provinces, China, February 2019

Euro Surveill. 2019 Jun;24(25):1900340. doi: 10.2807/1560-7917.ES.2019.24.25.1900340.

Authors

Li Bai^{1

2

3}, Pengcheng Du^{3

4}, Yinju Du⁵, Honghu Sun^{1

2

6}, Pei Zhang^{1

2}, Yuping Wan⁶, Qi Lin⁶, Séamus Fanning^{1

2

7}, Shenghui Cui⁸, Yongning Wu^{1

2}

Affiliations

¹ Key Laboratory of Food Safety Risk Assessment, National Health Commission of the People's Republic of China, Beijing, People's Republic of China.
² Food Safety Research Unit of Chinese Academy of Medical Sciences, China National Center for Food Safety Risk Assessment, Beijing, People's Republic of China.
³ These authors contributed equally to this work.
⁴ Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, and Beijing Key Laboratory of Emerging Infectious Diseases, Beijing, People's Republic of China.
⁵ Center for disease control and prevention of Liaocheng city, Liaocheng, People's Republic of China.
⁶ Chengdu institute for Food and Drug Control, Chengdu, People's Republic of China.
⁷ UCD-Centre for Food Safety, School of Public Health, Physiotherapy and Sports Science, University College Dublin, Belfield, Dublin, Ireland.
⁸ Department of Food Science, National Institutes for Food and Drug Control, Beijing, People's Republic of China.

PMID: 31241040
PMCID: PMC6593906
DOI: 10.2807/1560-7917.ES.2019.24.25.1900340

Abstract

The plasmid-mediated high-level tigecycline resistance gene, tet(X4), was detected in seven Escherichia coli isolates from pork in two Chinese provinces. Two isolates belonged to the epidemic spreading sequence type ST101. Tet(X4) was adjacent to ISVsa3 and concurrent with floR in all seven isolates. In addition to IncFIB, the replicon IncFII was found to be linked to tet(X4). This report follows a recent detection of tet(X3)/(X4) in E. coli from animals and humans in China.

Keywords: Escherichia coli; conjugative plasmid; tigecycline resistance.

MeSH terms

Animals
Anti-Bacterial Agents / pharmacology*
China
Drug Resistance, Bacterial
Electrophoresis, Gel, Pulsed-Field
Escherichia coli / drug effects*
Escherichia coli / genetics*
Escherichia coli / isolation & purification
Escherichia coli Infections / microbiology
Escherichia coli Infections / veterinary*
Escherichia coli Proteins / genetics
Escherichia coli Proteins / metabolism
Microbial Sensitivity Tests
Plasmids / genetics*
Plasmids / metabolism
Polymorphism, Single Nucleotide
Pork Meat / microbiology*
Protein Synthesis Inhibitors
Swine
Swine Diseases / microbiology*
Tetracyclines / metabolism
Tetracyclines / pharmacology
Tigecycline / metabolism
Tigecycline / pharmacology*

Substances

Anti-Bacterial Agents
Escherichia coli Proteins
Protein Synthesis Inhibitors
Tetracyclines
Tigecycline