CoGe LoadExp+: A web-based suite that integrates next-generation sequencing data analysis workflows and visualization

Plant Direct. 2017 Jul 20;1(2):10.1002/pld3.8. doi: 10.1002/pld3.8. eCollection 2017 Jul.

Abstract

To make genomic and epigenomic analyses more widely available to the biological research community, we have created LoadExp+, a suite of bioinformatics workflows integrated with the web-based comparative genomics platform, CoGe. LoadExp+ allows users to perform transcriptomic (RNA-seq), epigenomic (bisulfite-seq), chromatin-binding (ChIP-seq), variant identification (SNPs), and population genetics analyses against any genome in CoGe, including genomes integrated by users themselves. Through LoadExp+'s integration with CoGe's existing features, all analyses are available for visualization and additional downstream processing, and are available for export to CyVerse's data management and analysis platforms. LoadExp+ provides easy-to-use functionality to manage genomics and epigenomics data throughout its entire lifecycle using a publicly available web-based platform and facilitates greater accessibility of genomics analyses to researchers of all skill levels. LoadExp+ can be accessed at https://genomevolution.org.

Keywords: ChIP‐seq; DNA methylation; RNA‐seq; SNP identification; bisulfite sequencing; epigenomics; expression analysis; genomics; next‐generation sequencing; population genetics.