Glycogen synthase kinase 3β (GSK3β) regulates the expression of MyHC2a in goat skeletal muscle satellite cells (SMSCs)

Anim Sci J. 2019 Aug;90(8):1042-1049. doi: 10.1111/asj.13253. Epub 2019 Jun 24.

Abstract

Glycogen synthase kinase beta (GSK3β) plays an important role in skeletal muscle growth, regeneration, and repair. However, the mechanism of GSK3β regulating MyHC2a expression is currently not clear. In this study, GSK3β inhibition promoted skeletal muscle satellite cells (SMSCs) differentiation and increased expression of MyoD, MyoG, MyHC1, and MyHC2a genes. Then we cloned approximately 1.1 kb of goat MyHC2a gene promoter. The deletion fragment (-514/+55) of MyHC2a promoter exhibited the highest level of promoter activity, and a NFATc2 element in this region was responsible for MyHC2a promoter activity. Treatment of SB216713 significantly decreased the transcriptional activity of the fragment (-514/+55). Furthermore, GSK3β inhibition had no effect on the luciferase activity of MyHC2a promoter after mutating the NFATc2-binding site. These results demonstrated that GSK3β inhibition promoted SMSCs differentiation and regulated the MyHC2a gene expression through NFATc2 in goat-differentiated SMSCs.

Keywords: GSK3β; MyHC2a; goat; promoter; satellite cells.

MeSH terms

  • Animals
  • Cell Differentiation / genetics
  • Cells, Cultured
  • Female
  • Gene Expression / genetics*
  • Gene Expression Regulation, Developmental / genetics*
  • Glycogen Synthase Kinase 3 beta / antagonists & inhibitors*
  • Glycogen Synthase Kinase 3 beta / physiology*
  • Goats
  • Luciferases / metabolism
  • Muscle, Skeletal / cytology*
  • Myosin Heavy Chains / genetics*
  • Myosin Heavy Chains / metabolism*
  • NFATC Transcription Factors
  • Satellite Cells, Skeletal Muscle / metabolism*

Substances

  • NFATC Transcription Factors
  • Luciferases
  • Glycogen Synthase Kinase 3 beta
  • Myosin Heavy Chains