Continuous sulfur biotransformation in an anaerobic-anoxic sequential batch reactor involving sulfate reduction and denitrifying sulfide oxidization

Abstract

The pathways and intermediates of continuous sulfur biotransformation in an anaerobic and anoxic sequential batch reactor (AA-SBR) involving sulfate reduction (SR) and denitrifying sulfide oxidization (DSO) were investigated. In the anoxic phase, DSO occurred in two sequential steps, the oxidation of sulfide (S^2-) to elemental sulfur (S⁰) and the oxidation of S⁰ to sulfate (SO₄^2-). The oxidation rate of S^2- to S⁰ was 3.31 times faster than that of S⁰ to SO₄^2-, resulting in the accumulation of S⁰ as a desired intermediate under S^2--S/NO₃^--N ratio (molar ratio) of 0.9:1. Although, approximately 60% of generated S⁰ suspended in the effluent, about 40% of S⁰ retained in the sludge, which could be further oxidized or reduced in anoxic or anaerobic phase. In anoxic, S⁰ was subsequently oxidized to SO₄^2- under S^2--S/NO₃^--N ratio of 0.5:1. In anaerobic, S⁰ coexist with SO₄^2- (in fresh wastewater) were simultaneously reduced to S^2-, and the reduction rate of SO₄^2- to S^2- was 3.17 times faster than that of S⁰ to S^2-, resulting in a higher production of S⁰ in subsequent anoxic phase. Microbial community analysis indicated that SO₄^2-/S⁰-reducing bacteria (e.g. Desulfomicrobium and Desulfuromonas) and S^2-/S⁰-oxidizing bacteria (e.g. Paracoccus and Thermothrix) co-participated in continuous sulfur biotransformation in the AA-SBR. A conceptual model was established to describe these main processes and key intermediates. The research offers a new insight into the reaction processes optimization for S⁰ recovery and simultaneous removal of SO₄^2- and NO₃^- in an AA-SBR.

Keywords: Denitrifying sulfide oxidization; Elemental sulfur; Microbial community analysis; Sulfate reduction; Sulfur biotransformation.