Structure, antiproliferative and cancer preventive properties of sulfated α-d-fucan from the marine bacterium Vadicella arenosi

Maxim S Kokoulin; Alina P Filshtein; Lyudmila A Romanenko; Irina V Chikalovets; Oleg V Chernikov

doi:10.1016/j.carbpol.2019.05.086

Structure, antiproliferative and cancer preventive properties of sulfated α-d-fucan from the marine bacterium Vadicella arenosi

Carbohydr Polym. 2019 Oct 1:221:120-126. doi: 10.1016/j.carbpol.2019.05.086. Epub 2019 May 31.

Authors

Maxim S Kokoulin¹, Alina P Filshtein², Lyudmila A Romanenko², Irina V Chikalovets³, Oleg V Chernikov²

Affiliations

¹ G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 159/2, Prospect 100 let Vladivostoku, Vladivostok, 690022, Russia. Electronic address: maxchem@mail.ru.
² G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 159/2, Prospect 100 let Vladivostoku, Vladivostok, 690022, Russia.
³ G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Far Eastern Branch, Russian Academy of Sciences, 159/2, Prospect 100 let Vladivostoku, Vladivostok, 690022, Russia; Far Eastern Federal University, 8, Sukhanova str., Vladivostok, 690950, Russia.

PMID: 31227150
DOI: 10.1016/j.carbpol.2019.05.086

Abstract

Sulfated fucose-containing glycopolymers are currently of great interest because of their wide spectrum of bioactivity, including anti-tumor properties. In this study, the structure of O-polysaccharide (OPS) of the marine bacterium Vadicella arenosi KMM 9024^T, its effect on the proliferation of human breast cancer MCF-7 cells and cancer preventive properties were investigated. Two OPS fractions with different molecular weights were isolated and purified from the lipopolysaccharide by mild acid hydrolysis followed by anion-exchange chromatography. The OPS was found to consist of α-(1→3)-linked 2-O-sulfate-d-fucopyranosyl residues, whose structure was deduced by sugar analysis along with 2D NMR spectroscopy. The biological assay indicated that polysaccharide significantly reduced the proliferation and inhibited colony formation of MCF-7 cells in a dose-dependent manner. Besides, the experiment indicated the inhibitory role of polysaccharide on EGF-induced neoplastic cell transformation in mouse epidermal cells. The investigated polysaccharide is the first sulfated fucan isolated from the bacteria.

Keywords: Antiproliferative activity; Cancer preventive properties; Marine bacterium; O-polysaccharide; Sulfated fucan; Vadicella arenosi.

MeSH terms

Animals
Antineoplastic Agents / chemistry
Antineoplastic Agents / isolation & purification
Antineoplastic Agents / pharmacology*
Carbohydrate Sequence
Cell Proliferation / drug effects
Galactans / chemistry
Galactans / isolation & purification
Galactans / pharmacology*
Humans
MCF-7 Cells
Mice
Rhodobacteraceae / chemistry*
Sulfuric Acid Esters / chemistry
Sulfuric Acid Esters / isolation & purification
Sulfuric Acid Esters / pharmacology*

Substances

Antineoplastic Agents
Galactans
Sulfuric Acid Esters