Identification and characterization of cresol degrading Pseudomonas monteilii strain SHY from Soil samples

Shainy Nhattuketty Krishnan; Anuraj Nayarisseri; Usha Rajamanickam

doi:10.6026/97320630014455

Identification and characterization of cresol degrading Pseudomonas monteilii strain SHY from Soil samples

Bioinformation. 2018 Oct 3;14(9):455-464. doi: 10.6026/97320630014455. eCollection 2018.

Authors

Shainy Nhattuketty Krishnan^{1

2}, Anuraj Nayarisseri^{3

4}, Usha Rajamanickam¹

Affiliations

¹ Department of Microbiology, Karpagam University, Karpagam academy of higher education, Eachinary, Coimbatore - 641 021, Tamilnadu, India.
² Department of Microbiology, Safi center for scientific research, Vazhayoor East, Malappuram- 673 633, Kerala, India.
³ In silico Research Laboratory, Eminent Biosciences, Vijaynagar, Indore - 452010, Madhya Pradesh, India.
⁴ Bioinformatics Research Laboratory, LeGene Biosciences Pvt Ltd, Indore - 452010, Madhya Pradesh, India.

Abstract

Cresol is an organic pollutant discharged by pharmaceutical, pesticide, coal and gasification industries causing severe organ failure in humans. Therefore, it is of interest to isolate microbes from contaminated site for degrading cresol. We isolated a strain (CR-13) that survives at 5000ppm cresol with about 80 percent cresol degradation ability. Immobilized cells showed >99 percent degradation at high concentration of cresol. The 16S rRNA sequence (accession number: MF278026) deposited in GenBank was used for phylogenetic tree analysis and the strain was grouped under Pseudomonas monteilii. The isolated cresol degrading strain was subsequently named as Pseudomonas monteilii SHY.

Keywords: 16S rRNA Gene Sequencing; Cresol degrading bacteria; Pseudomonas monteilii.