OsCPK21 is required for pollen late-stage development in rice

Kexin Wen; Yixing Chen; Xiaojin Zhou; Shu Chang; Hao Feng; Jing Zhang; Zhilin Chu; Xiaogang Han; Jie Li; Jin Liu; Chao Xi; Heping Zhao; Shengcheng Han; Yingdian Wang

doi:10.1016/j.jplph.2019.153000

OsCPK21 is required for pollen late-stage development in rice

J Plant Physiol. 2019 Sep:240:153000. doi: 10.1016/j.jplph.2019.153000. Epub 2019 Jun 14.

Authors

Kexin Wen¹, Yixing Chen², Xiaojin Zhou³, Shu Chang⁴, Hao Feng⁵, Jing Zhang⁶, Zhilin Chu⁷, Xiaogang Han⁸, Jie Li⁹, Jin Liu¹⁰, Chao Xi¹¹, Heping Zhao¹², Shengcheng Han¹³, Yingdian Wang¹⁴

Affiliations

¹ Beijing Key Laboratory of Gene Resources and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing, 100875, China. Electronic address: 48722363@qq.com.
² Beijing Key Laboratory of Gene Resources and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing, 100875, China. Electronic address: 190976592@qq.com.
³ Beijing Key Laboratory of Gene Resources and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing, 100875, China; Department of Crop Genomic & Genetic Improvement, Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. Electronic address: zhouxiaojin@caas.cn.
⁴ Beijing Key Laboratory of Gene Resources and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing, 100875, China. Electronic address: 670310185@qq.com.
⁵ Beijing Key Laboratory of Gene Resources and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing, 100875, China. Electronic address: 214085929@qq.com.
⁶ Beijing Key Laboratory of Gene Resources and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing, 100875, China. Electronic address: 1036802706@qq.com.
⁷ Beijing Key Laboratory of Gene Resources and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing, 100875, China. Electronic address: zhiriyulin@sina.com.
⁸ Beijing Key Laboratory of Gene Resources and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing, 100875, China. Electronic address: 526793194@qq.com.
⁹ Beijing Key Laboratory of Gene Resources and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing, 100875, China. Electronic address: 78947505@qq.com.
¹⁰ Beijing Key Laboratory of Gene Resources and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing, 100875, China. Electronic address: liujin@bnu.edu.cn.
¹¹ Beijing Key Laboratory of Gene Resources and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing, 100875, China. Electronic address: xichao@bnu.edu.cn.
¹² Beijing Key Laboratory of Gene Resources and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing, 100875, China. Electronic address: hpzhao@bnu.edu.cn.
¹³ Beijing Key Laboratory of Gene Resources and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing, 100875, China. Electronic address: schan@bnu.edu.cn.
¹⁴ Beijing Key Laboratory of Gene Resources and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing, 100875, China. Electronic address: ydwang@bnu.eu.cn.

PMID: 31220626
DOI: 10.1016/j.jplph.2019.153000

Abstract

In flowering plants, pollen development is a critical step for reproductive success and necessarily involves complex genetic regulatory networks. Calcium-dependent protein kinases (CPKs) are plant-specific calcium sensors involved in the regulation of plant development and adaption to the environment; however, whether they play a role in regulating male reproduction remains elusive. Here, we found that the knockdown of spikelet-specific OsCPK21 causes pollen abortion in OsCPK21-RNAi transgenic plants. Severe defects in pollen development initiated at stage 10 of anther development and simultaneous cell death occurred in the pollen cells of OsCPK21-RNAi plants. Microarray analysis and qRT-PCR revealed that the transcription of OsCPK21 is coordinated with that of MIKC*-type MADS box transcription factors OsMADS62, OsMADS63, and OsMADS68 during rice anther development. We further showed that OsCPK21 indirectly up-regulates the transcription of OsMADS62, OsMADS63, and OsMADS68 through the potential MYB binding site, DRE/CRT element, and/or new ERF binding motif localised in the promoter region of these three MADS genes. These findings suggest that OsCPK21 plays an essential role in pollengenesis, possibly via indirectly regulating the transcription of MIKC*-type MADS box proteins.

Keywords: CPK21; Indirectly regulation; MIKC*-type MADS box proteins; Pollen development; Rice.

MeSH terms

Gene Expression Regulation, Plant*
Oryza / genetics*
Oryza / growth & development
Oryza / metabolism
Plant Proteins / genetics*
Plant Proteins / metabolism
Plants, Genetically Modified / genetics
Plants, Genetically Modified / growth & development
Plants, Genetically Modified / metabolism
Pollen / genetics
Pollen / growth & development
Protein Kinases / genetics*
Protein Kinases / metabolism
RNA Interference

Substances

Plant Proteins
Protein Kinases
calcium-dependent protein kinase