Titanium implants easily suffer bacteria-related infections in clinic due to their inherent lack of self-protection ability. Therefore, a novel Ti-Cu alloy with good antibacterial activity has been developed as a new kind of implant material. This study focuses on a systematic evaluation of both cytocompatibility and osteogenesis activity of the Ti-Cu alloy in vitro. It was revealed that an addition of 5% Cu into pure Ti would not cause any negative effect on osteoblasts adhesion, proliferation and apoptosis cultured with Ti-Cu alloy. In addition, Ti-Cu alloy could significantly promote the osteogenic differentiation of MG 63 cells by upregulating the osteogenesis-related gene expressions including alkaline phosphatase (ALP), Collagen I (Colla I), osteopontin (OPN) and osteocalcin (OCN). These promising results suggest that the Ti-Cu alloy has great potential to be used as a multi-functional titanium implant for clinical applications.