Multiplex optical detection and quantification of DNA fragments by metallo-peptide assemblies

Abhijit Saha; Meital Reches

doi:10.1038/s41598-019-45124-z

Multiplex optical detection and quantification of DNA fragments by metallo-peptide assemblies

Sci Rep. 2019 Jun 19;9(1):8789. doi: 10.1038/s41598-019-45124-z.

Authors

Abhijit Saha^{1

2}, Meital Reches^{3

4}

Affiliations

¹ Institute of Chemistry, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Givat Ram, Jerusalem, 9190401, Israel.
² The center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem, 9190401, Israel.
³ Institute of Chemistry, The Hebrew University of Jerusalem, Edmond J. Safra Campus, Givat Ram, Jerusalem, 9190401, Israel. meital.reches@mail.huji.ac.il.
⁴ The center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem, 9190401, Israel. meital.reches@mail.huji.ac.il.

Abstract

Rapid detection of infectious agents such as bacteria and viruses are important for proper health management, agriculture and homeland security. This paper presents a multiplex DNA detection system self-assembled by a metallo-peptide complex. Within five minutes, the system can simultaneously detect multiple DNA fragments, without any need for their separation. The presence of proteins in the sample does not harm the detection capabilities of the system, which can discriminate even between one base-pair mismatch and can perform at concentrations as low as 200 pM.

MeSH terms

Calibration
DNA / analysis*
Fluorescence
Limit of Detection
Metals / chemistry*
Optical Imaging*
Peptides / chemistry*

Substances

Metals
Peptides
DNA