DNAzyme-Functionalized R-Phycoerythrin as a Cost-Effective and Environment-Friendly Fluorescent Biosensor for Aqueous Pb2+ Detection

Jikui Wu; Yunfei Lu; Ningna Ren; Min Jia; Ruinan Wang; Junling Zhang

doi:10.3390/s19122732

DNAzyme-Functionalized R-Phycoerythrin as a Cost-Effective and Environment-Friendly Fluorescent Biosensor for Aqueous Pb²⁺ Detection

Sensors (Basel). 2019 Jun 18;19(12):2732. doi: 10.3390/s19122732.

Authors

Jikui Wu¹, Yunfei Lu², Ningna Ren³, Min Jia⁴, Ruinan Wang⁵, Junling Zhang⁶

Affiliations

¹ College of Food Science and Technology, Ministry of Agriculture, National R&D Branch Center for Freshwater Aquatic Products Processing Technology (Shanghai), Laboratory of Quality and Safety Risk Assessment for Aquatic Product on Storage and Preservation (Shanghai), Shanghai Ocean University, Shanghai 201306, China. jkwu@shou.edu.cn.
² College of Food Science and Technology, Ministry of Agriculture, National R&D Branch Center for Freshwater Aquatic Products Processing Technology (Shanghai), Laboratory of Quality and Safety Risk Assessment for Aquatic Product on Storage and Preservation (Shanghai), Shanghai Ocean University, Shanghai 201306, China. yifland0929@163.com.
³ College of Food Science and Technology, Ministry of Agriculture, National R&D Branch Center for Freshwater Aquatic Products Processing Technology (Shanghai), Laboratory of Quality and Safety Risk Assessment for Aquatic Product on Storage and Preservation (Shanghai), Shanghai Ocean University, Shanghai 201306, China. Ningna_ren@163.com.
⁴ College of Food Science and Technology, Ministry of Agriculture, National R&D Branch Center for Freshwater Aquatic Products Processing Technology (Shanghai), Laboratory of Quality and Safety Risk Assessment for Aquatic Product on Storage and Preservation (Shanghai), Shanghai Ocean University, Shanghai 201306, China. m170200397@st.shou.edu.cn.
⁵ College of Food Science and Technology, Ministry of Agriculture, National R&D Branch Center for Freshwater Aquatic Products Processing Technology (Shanghai), Laboratory of Quality and Safety Risk Assessment for Aquatic Product on Storage and Preservation (Shanghai), Shanghai Ocean University, Shanghai 201306, China. m170200399@st.shou.edu.cn.
⁶ Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Engineering Research Center of Aquaculture, National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China. jlzhang@shou.edu.cn.

Abstract

The sensitive detection of Pb²⁺ is of significant importance for food safety, environmental monitoring, and human health care. To this end, a novel fluorescent biosensor, DNAzyme-functionalized R-phycoerythrin (DNAzyme-R-PE), was presented for Pb²⁺ analysis. The biosensor was prepared via the immobilization of Iowa Black^® FQ-modified DNAzyme-substrate complex onto the surface of SPDP-functionalized R-PE. The biosensor produced a minimal fluorescence signal in the absence of Pb²⁺. However, Pb²⁺ recognition can induce the cleavage of substrate, resulting in a fluorescence restoration of R-PE. The fluorescence changes were used to measure sensitively Pb²⁺ and the limit of detection was 0.16 nM with a linear range from 0.5-75 nM. Furthermore, the proposed biosensor showed excellent selectivity towards Pb²⁺ even in the presence of other metal ions interferences and was demonstrated to successfully determine Pb²⁺ in spiked lake water samples.

Keywords: DNAzyme; Pb2+; R-phycoerythrin; fluorescence resonance energy transfer; sensors.

MeSH terms

Biosensing Techniques*
DNA, Catalytic / chemistry*
Environmental Monitoring
Fluorescence
Food Safety
Lead / chemistry
Lead / isolation & purification*
Phycoerythrin / chemistry*

Substances

DNA, Catalytic
Phycoerythrin
Lead