Epstein-Barr virus (EBV) initiates various immune disorders, including changes in cytokine profile, which result in the development of different pathological conditions, in particular, allergopathology. Aim - to investigate the level of cytokines with antiviral activity in patients with allergopathology in active and latent phases of chronic persistence of Epstein-Barr viral infection. In general, 38 patients with allergopathology in combination with chronic persistence of EBV-infection in active (1st group) and latent (2nd group) phases were examined. A complex clinical and laboratory examination was performed; immunological, molecular and genetic, instrumental investigations, statistical analysis of the results were conducted. Peculiarities of the levels of cytokines with antiviral activity IL12, IL1β, IL33, TNF-α, IFN-g were investigated in two groups of patients: 1st group - patients with allergy in combination with active phase (PCR "+") of chronic EBV infection, 2nd group - patients with allergy in combination with latent phase (PCR "-") of chronic EBV infection. Control group - 20 healthy individuals of corresponding gender and age. In patients with allergopathology in combination with chronic persistence of EBV, marked deregulation of optimal correlation of cytokine network components was detected, in particular, elevation of IL1β concentration with simultaneous decrease in IL12, IL33 and normal level of IFN-g and TNF-α (in active phase) and reduction of IL1β, IFN-g concentration with simultaneous increase in IL12, IL33 and normal level of TNF-α (in latent phase) of infection. Absence of consolidated influence of investigated cytokines with antiviral activity plays a leading role in pathogenesis of pathological process under conditions of continuous persistence of the virus in the body with its further activation. The obtained proofs of cytokine imbalance indicate inadequate cell-mediated and humoral response of the body in chronic EBV-infection with allergopathology formation.