Use of a Monoclonal Antibody and Two Enzyme-Linked Immunosorbent Assay Formats for Detection and Quantification of the Substitution of Caprine Milk for Ovine Milk

Ana I Haza; Paloma Morales; Rosario Martíin; Teresa Garcíia; Gonzalo Anguita; Isabel González; Bernabé Sanz; Pablo E Hernández

doi:10.4315/0362-028X-60.8.973

Use of a Monoclonal Antibody and Two Enzyme-Linked Immunosorbent Assay Formats for Detection and Quantification of the Substitution of Caprine Milk for Ovine Milk

J Food Prot. 1997 Aug;60(8):973-977. doi: 10.4315/0362-028X-60.8.973.

Authors

Ana I Haza¹, Paloma Morales¹, Rosario Martíin¹, Teresa Garcíia¹, Gonzalo Anguita¹, Isabel González¹, Bernabé Sanz¹, Pablo E Hernández¹

Affiliation

¹ Departamento de Nutrición y Bromatología III, Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain.

PMID: 31207812
DOI: 10.4315/0362-028X-60.8.973

Abstract

A stable hybridoma cell line (B2B) has been produced that secretes a monoclonal antibody (MAb) specific for goat's milk α_S2-casein. The MAb B2B was used in two enzyme-linked immunosorbent assay (ELISA) formats for the detection and quantification of the presence of goat's milk in ewe's milk. In the indirect ELISA format the limit of detection was 0.5 to 15% (vol/vol) substitution of goat's milk for ewe's milk. Afterwards, a competitive indirect ELISA was successfully developed for the detection of 0.25 to 15% (vol/vol) of goat's milk in ewe's milk. This competitive indirect ELISA is a very sensitive assay; it can be performed in less than 5 h and is not influenced by the heat treatment of milk.

Keywords: Monoclonal antibody; competitive indirect ELISA; goat's milk detection; indirect ELISA.