Oat protein concentrate as alternative ingredient for non-dairy yoghurt-type product

J Sci Food Agric. 2019 Oct;99(13):5852-5857. doi: 10.1002/jsfa.9858. Epub 2019 Jul 29.

Abstract

Background: During the industrial production of β-glucan, a protein-rich fraction remains as a by-product. Recovery of this protein as oat protein concentrate (OPC) results in a source of cereal protein for food and improves the overall economy of the process. In this study, a yoghurt-type product is developed by lactic acid fermentation of an OPC suspension after subjection to heat treatment to assure starch gelatinization.

Results: In detail, the process of yoghurt production involved an initial heating step to 90 °C, followed by 24 h fermentation with a starter culture consisting of Lactobacillus delbrueckii subsp. bulgaricus und Streptococcus thermophilus. The resulting yoghurt-type product was mildly sour (pH 4.2) with a certain amount of lactic acid (3.3 ± 0.2 g kg-1 ) and contained 4.9 × 106 cfu g-1 lactobacillus after 24 h fermentation. Scanning electron microscopy revealed a porous network presumably built up from the gelatinized starch fraction containing aggregated structures, between which were assumed to be aggregated oat proteins. Moreover, to a limited extent, proteolysis occurred during fermentation. Thus some of the proteolytic enzymes present in the yoghurt culture cleaved oat protein and released peptides. However, the effect on essential amino acids was small.

Conclusion: The results of this study provide a deeper knowledge into the role of starch and protein in fermented OPC yoghurts. The structure of fermented OPC verifies the applicability of oat protein as an alternative source for yoghurt-type products. © 2019 Society of Chemical Industry.

Keywords: lactic acid fermentation; non-dairy yoghurt; oat protein concentrate; plant proteins.

Publication types

  • Evaluation Study

MeSH terms

  • Amino Acids, Essential / analysis
  • Amino Acids, Essential / metabolism
  • Avena / chemistry
  • Avena / metabolism*
  • Avena / microbiology
  • Fermentation
  • Food Additives / analysis*
  • Food Additives / metabolism
  • Food Handling / methods*
  • Lactic Acid / analysis
  • Lactic Acid / metabolism
  • Lactobacillus delbrueckii / metabolism*
  • Plant Proteins / analysis*
  • Plant Proteins / metabolism
  • Streptococcus thermophilus / metabolism*
  • Yogurt / analysis*
  • Yogurt / microbiology

Substances

  • Amino Acids, Essential
  • Food Additives
  • Plant Proteins
  • Lactic Acid