Objective: To investigate the expression of miR-101 and EZH2 in patients with mantle cell lymphoma(MCL) and to analyze its correlation with clinical prognosis of MCL patients.
Methods: RQ-PCR and S-P immunohistochemistry were used to detect the expressions of miR-101 and EZH2 in tissue of MCL patients. CCK-8 was used to assay the effect of miR-100 minics on the proliferation of Jeko-1 and Mino cells; the flow cytometry with Annexin V/PI double staining was used to assay the apoptosis; Western blot was used to assay the effect of miR-101 minics on the expression of EZH2 protein in Jeko-1 and Mino cells.
Results: Compared with control group, miR-101 lowly expressed, and EZH2 protein highly expressed in MCL group, with very statistically significant difference(P<0.01).There was negative correlation between miR-101 and EZH2 expression(r=-0.638，P<0.05). The expression of miR-101 and EZH2 significantly correlated with B symptoms, International Prognostic Index(IPI) and Ann Arbor stage, respectively. Survival analysis showed that the overall survival(OS) rate of patients with low expression of miR-101 were significantly lower than that of patients with high miR-101 expression (P=0.0014), the OS rate of patients with EZH2 high expression were significantly lower than that of patients with EZH2 low expression (P=0.0093). The miR-100 minics could inhibit the proliferation of Jeko-1 and Mino cells, and increase the apoptotic rate. The expression of EZH2 protein was markedly suppressed by the miR-100 minics.
Conclusion: The expression of miR-101 and EZH2 is different in MCL patients with different clinical stage and prognosis. The miR-101 can inhibit the cell proliferation and induce cell apoptosis of mantle cell lymphoma by targeting EZH2.
题目: miR-101和EZH2在套细胞淋巴瘤中的表达及其临床意义.
目的: ]探讨miR-101和EZH2在套细胞淋巴瘤（mantle cell lymphoma, MCL）中的表达及其临床意义.
方法: 应用RQ-PCR、免疫组织化学S-P法检测MCL患者淋巴结病理组织中miR-101和EZH2蛋白表达水平。比较miR-101、EZH2不同表达水平的MCL患者的生存情况。 应用CCK-8检测miR-101 minics对MCL的Jeko-1、Mino细胞增殖的影响，AnnexinⅤ/PI双染流式细胞术检测miR-101 minics对Jeko-1、Mino细胞凋亡的影响，Western blot检测miR-101 minics对Jeko-1、Mino细胞EZH2蛋白的表达水平的作用.
结果: 与对照组相比，MCL组miR-101低表达，而EZH2蛋白高表达，差异均有统计学意义(P<0.01)。Spearman等级相关分析显示，miR-101与EZH2蛋白表达水平存在负相关（r=-0.638，P<0.05）。miR-101、EZH2表达水平与MCL患者B症状、IPI评分、AnnArbor临床分期存在相关性(P<0.05)。miR-101低表达组患者生存率显著低于高表达患者（P=0.0014），而EHZ2高表达组患者生存率显著低于低表达患者(P=0.0093)。 转染miR-100 minics 后，Jeko-1、Mino细胞的EZH2蛋白表达下降，Jeko-1、Mino细胞增殖率降低，而细胞凋亡率增加.
结论: miR-101和EZH2在不同临床分期和预后的MCL患者淋巴组织中表达存在差异，miR-101靶向EZH2基因表达能抑制MCL细胞增殖，诱导细胞凋亡.