On the evaluation of different saccharification schemes for enhanced bioethanol production from potato peels waste via a newly isolated yeast strain of Wickerhamomyces anomalus

Imen Ben Atitallah; Georgia Antonopoulou; Ioanna Ntaikou; Maria Alexandropoulou; Moncef Nasri; Tahar Mechichi; Gerasimos Lyberatos

doi:10.1016/j.biortech.2019.121614

On the evaluation of different saccharification schemes for enhanced bioethanol production from potato peels waste via a newly isolated yeast strain of Wickerhamomyces anomalus

Bioresour Technol. 2019 Oct:289:121614. doi: 10.1016/j.biortech.2019.121614. Epub 2019 Jun 7.

Authors

Imen Ben Atitallah¹, Georgia Antonopoulou², Ioanna Ntaikou³, Maria Alexandropoulou³, Moncef Nasri⁴, Tahar Mechichi⁵, Gerasimos Lyberatos⁶

Affiliations

¹ Laboratory of Enzyme Engineering and Microbiology, National School of Engineers of Sfax, University of Sfax, BP 1173, 3038 Sfax, Tunisia; Laboratory of Biochemistry and Enzymatic Engineering of Lipases, National School of Engineers of Sfax, University of Sfax, 3038 Sfax, Tunisia.
² Institute of Chemical Engineering Sciences, Stadiou, Platani, Patras GR 26504, Greece. Electronic address: geogant@chemeng.upatras.gr.
³ Institute of Chemical Engineering Sciences, Stadiou, Platani, Patras GR 26504, Greece.
⁴ Laboratory of Enzyme Engineering and Microbiology, National School of Engineers of Sfax, University of Sfax, BP 1173, 3038 Sfax, Tunisia.
⁵ Laboratory of Biochemistry and Enzymatic Engineering of Lipases, National School of Engineers of Sfax, University of Sfax, 3038 Sfax, Tunisia.
⁶ Institute of Chemical Engineering Sciences, Stadiou, Platani, Patras GR 26504, Greece; School of Chemical Engineering, National Technical University of Athens, GR 15780 Athens, Greece.

PMID: 31203181
DOI: 10.1016/j.biortech.2019.121614

Abstract

The present study focuses on the exploration of the potential use of potato peels waste (PPW) as feedstock for bioethanol production, using a newly isolated yeast strain, Wickerhamomyces anomalus, via different saccharification and fermentation schemes. The saccharification of PPW was performed via thermal and chemical (acid, alkali) pretreatment, as well as via enzymatic hydrolysis through the use of commercial enzymes (cellulase and amylase) or enzymes produced at lab scale (alpha-amylase from Bacillus sp. Gb67), either separately or in mixtures. The results indicated that the enzymatic treatment by commercial enzymes led to a higher saccharification efficiency (72.38%) and ethanol yield (0.49 g/g_{consumed sugars}) corresponding to 96% of the maximum theoretical. In addition, acid pretreatment was found to be beneficial for the process, leading also to high hydrolysis and ethanol yields, indicating that PPW is a very promising feedstock for bio-ethanol production by W. anomalus under different process schemes.

Keywords: Bioethanol; Enzymatic saccharification; Potato peels waste; Pretreatment; W. anomalus.

MeSH terms

Cellulase*
Ethanol
Fermentation
Hydrolysis
Saccharomyces cerevisiae
Solanum tuberosum*

Substances

Ethanol
Cellulase