Acute, genetic and sub-chronic toxicities of flaxseed derived Maillard reaction products

Chao-Kun Wei; Zhi-Jing Ni; Kiran Thakur; Ai-Mei Liao; Fei Hu; Ji-Hong Huang; Zhao-Jun Wei

doi:10.1016/j.fct.2019.110580

Acute, genetic and sub-chronic toxicities of flaxseed derived Maillard reaction products

Food Chem Toxicol. 2019 Sep:131:110580. doi: 10.1016/j.fct.2019.110580. Epub 2019 Jun 13.

Authors

Chao-Kun Wei¹, Zhi-Jing Ni², Kiran Thakur³, Ai-Mei Liao⁴, Fei Hu⁵, Ji-Hong Huang⁶, Zhao-Jun Wei⁷

Affiliations

¹ School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009, China. Electronic address: weichaokun2014@163.com.
² School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009, China; Biological Science and Engineering College, North Minzu University, Yinchuan, 750021, China. Electronic address: lovebear@vip.163.com.
³ School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009, China. Electronic address: kumarikiran@hfut.edu.cn.
⁴ College of Biological Engineering, Henan University of Technology, Zhengzhou, 450001, China. Electronic address: aimeiliao@haut.edu.cn.
⁵ School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009, China. Electronic address: hufei@hfut.edu.cn.
⁶ College of Biological Engineering, Henan University of Technology, Zhengzhou, 450001, China; Henan Cooperation Science and Technology Institute, Luoyang, 471000, China. Electronic address: huangjihong@haut.edu.cn.
⁷ School of Food and Biological Engineering, Hefei University of Technology, Hefei, 230009, China; Anhui Province Key Laboratory of Functional Compound Seasoning, Anhui Qiangwang Seasoning Food Co., Ltd., Jieshou, 236500, China. Electronic address: zjwei@hfut.edu.cn.

PMID: 31202938
DOI: 10.1016/j.fct.2019.110580

Abstract

In present study, the acute, genetic, and sub-chronic toxicities of flaxseed derived Maillard reaction products (MRPs) were investigated. Acute toxicity results showed that the 50% lethal dose (LD₅₀) of MRPs in rats was >15.0 g/kg body weight (BW); whereas, the 50% effective dose (ED₅₀) of MRPs was 12.3 g/kg BW. Ames test demonstrated that the back-mutation colonies for MRPs addition of 5,000 μg/dish was positive, which displayed certain mutagenicity. There were no significant differences in micronucleus rate and sperm deformity rate among different dose groups. The sub-chronic toxicity confirmed that less than 0.75 gMRPs/kg BW intake did not affect weight, food intake, mortality, gross pathology, histology, hematology, and serum biochemistry. The obtained results can provide an imperative reference on the safety of a meat flavoring agents.

Keywords: Acute toxicity; Flaxseed; Genetic toxicity; Maillard reaction products; Safety; Sub-chronic toxicity.

MeSH terms

Alanine Transaminase / metabolism
Animals
Bone Marrow / pathology
Female
Flax / chemistry*
Glycation End Products, Advanced / toxicity*
Kidney / pathology
Liver / pathology
Male
Mice
Mutagenicity Tests
Mutagens / toxicity
Rats, Sprague-Dawley
Seeds / chemistry*
Toxicity Tests, Acute
Toxicity Tests, Subchronic

Substances

Glycation End Products, Advanced
Mutagens
Alanine Transaminase