A pyrazole-containing hydrazone for fluorescent imaging of Al3+ in lysosomes and its resultant Al3+ complex as a sensor for F

Talanta. 2019 Oct 1:203:178-185. doi: 10.1016/j.talanta.2019.05.051. Epub 2019 May 14.

Abstract

A fluorescence-enhanced and lysosome-targeted Al3+ probe (1) based on a pyrazole-containing hydrazone was developed in this work. The fluorescent probe 1 displays an OFF-ON fluorescence response to Al3+ in the lysosome pH environment. In addition, the resultant 1-Al3+ complex could act as an ON-OFF fluorescence sensor for F-. Fluorescence imaging shows that 1 is suitable for visualization of Al3+ in lysosomes of HeLa cells.

Keywords: Al(3+); Cell imaging; F(−); Fluorescent probe; Hydrazone; Lysosome.

MeSH terms

  • Aluminum / analysis*
  • Coordination Complexes / analysis*
  • Density Functional Theory
  • Fluorescent Dyes / chemical synthesis
  • Fluorescent Dyes / chemistry*
  • Fluorides / analysis*
  • HeLa Cells
  • Humans
  • Hydrazones / chemical synthesis
  • Hydrazones / chemistry*
  • Lysosomes / metabolism*
  • Models, Chemical
  • Pyrazoles / chemical synthesis
  • Pyrazoles / chemistry*
  • Spectrometry, Fluorescence / methods

Substances

  • Coordination Complexes
  • Fluorescent Dyes
  • Hydrazones
  • Pyrazoles
  • Aluminum
  • Fluorides