Sources of variation evaluation of 24,25(OH)2D levels and the ratio of 25OHD to 24,25(OH)2D in apparently healthy Chinese adults: a multicenter cross-sectional study

Abstract

24,25(OH)₂D and the 25OHD/24,25(OH)₂D ratio have recently been shown to be useful for screening for hypercalcemia caused by CYP24A1 mutations. However, no population-based data on 24,25(OH)₂D have been published for Chinese populations, and few studies evaluated the sources of variation on 24,25(OH)₂D levels and the 25OHD/24,25(OH)₂D ratio. Hence, in 2018, we enrolled 1211 apparently healthy adults without systematic diseases from six representative cities in China (Beijing, Dongying, Guiyang, Urumqi, Shenzhen, and Qiqihar). 24,25(OH)₂D and 25OHD levels were measured using isotope dilution liquid chromatography tandem mass spectrometry. Multiple regression analysis showed that sex contributed the most to variations in 24,25(OH)₂D, 25OHD, and 25OHD/24,25(OH)₂D (rp= -0.255, -0.253, and 0.141, respectively), and age also contributed to variations in 25OHD but not 24,25(OH)₂D or 25OHD/24,25(OH)₂D. Men had significantly higher 24,25(OH)₂D and 25OHD levels than women, but had significantly lower 25OHD/24,25(OH)₂D values. Individuals from Dongying had the highest 24,25(OH)₂D and 25OHD values, whereas individuals from Urumqi had the lowest values. The median(2.5-97.5%) values for 24,25(OH)₂D, 25OHD, and 25OHD/24,25(OH)₂D were 1.2(0.36-2.65) ng/mL, 18.9(8.6-32.5) ng/mL, and 16.0 (9.8-30.8), respectively. 24,25(OH)₂D was significantly correlated with 25OHD (r = 0.838, p < 0.001), and 25OHD/24,25(OH)₂D was significantly negatively correlated with 24,25(OH)₂D (r = -0.758, P < 0.001) and 25OHD (r = -0.310, P < 0.001). In conclusion, in this nationwide, multicenter, cross-sectional study, we evaluated the levels of 24,25(OH)₂D and the 25OHD/24,25(OH)₂D ratio in the Chinese population. Sex contributed the most to variations in 24,25(OH)₂D, 25OHD, and 25OHD/24,25(OH)₂D.

Keywords: 24,25-dihydroxyvitamin D; 25-hydroxyvitamin D; Multicenter study.