Caged luciferins for bioluminescent activity-based sensing

Timothy A Su; Kevin J Bruemmer; Christopher J Chang

doi:10.1016/j.copbio.2019.05.002

Caged luciferins for bioluminescent activity-based sensing

Curr Opin Biotechnol. 2019 Dec:60:198-204. doi: 10.1016/j.copbio.2019.05.002. Epub 2019 Jun 11.

Authors

Timothy A Su¹, Kevin J Bruemmer¹, Christopher J Chang²

Affiliations

¹ Department of Chemistry, University of California, Berkeley, CA 94720, United States.
² Department of Chemistry, University of California, Berkeley, CA 94720, United States; Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, United States; Helen Wills Neuroscience Institute, University of California, Berkeley, CA 94720, United States; Howard Hughes Medical Institute, University of California, Berkeley, California 94720, United States.

Abstract

Bioluminescence imaging is a powerful modality for in vivo imaging owing to its low background and high signal-to-noise ratio. Because bioluminescent emission occurs only upon the catalytic reaction between the luciferase enzyme and its luciferin substrate, caging luciferins with analyte-reactive triggers offers a general approach for activity-based sensing of specific biochemical processes in living systems across cell, tissue, and animal models. In this review, we summarize recent efforts in the development of synthetic caged luciferins for tracking enzyme, small molecule, and metal ion activity and their contributions to physiological and pathological processes.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Review

MeSH terms

Animals
Luciferases
Luminescent Measurements*

Substances

Luciferases

Abstract

Publication types

MeSH terms

Substances

Grants and funding