We report the development and initial operation of a 6.4 meter perimeter laser gyroscope employing the 543.3 nm, 3s2→2p10 transition of neon. Employing fused silica based supermirrors yielding a cavity Q of 3.9×1011 and an inferred lock in threshold below 1 μHz, the gyroscope unlocked on the bias provided by Earth rotation alone with a measured Sagnac frequency of approximately 133 Hz, which is 16% larger than that of the 632.8 nm transition.