Construction of DNA Barcode-Tagged Salmonella Strains

Yichao Yang; Reena Chandrashekar; Steven C Ricke; Young Min Kwon

doi:10.1007/978-1-4939-9570-7_13

Construction of DNA Barcode-Tagged Salmonella Strains

Methods Mol Biol. 2019:2016:141-150. doi: 10.1007/978-1-4939-9570-7_13.

Authors

Yichao Yang¹, Reena Chandrashekar¹, Steven C Ricke^{2

3}, Young Min Kwon^{4

5}

Affiliations

¹ Department of Poultry Science, University of Arkansas, Fayetteville, AR, USA.
² Department of Food Science, Center for Food Safety, University of Arkansas, Fayetteville, AR, USA.
³ Cell and Molecular Biology Program, University of Arkansas, Fayetteville, AR, USA.
⁴ Department of Poultry Science, University of Arkansas, Fayetteville, AR, USA. ykwon@uark.edu.
⁵ Cell and Molecular Biology Program, University of Arkansas, Fayetteville, AR, USA. ykwon@uark.edu.

PMID: 31197716
DOI: 10.1007/978-1-4939-9570-7_13

Abstract

This chapter provides a detailed protocol for construction of DNA barcode-tagged isogenic strains of Salmonella. The protocol is illustrated with S. Enteritidis in this chapter. However, this protocol should be widely applicable to other Salmonella serotypes. A series of the DNA barcode-tagged strains thus constructed can be used in combination with next generation sequencing or quantitative PCR to study the population dynamics of the bacterial pathogen during infection within the host or transmission within a population of the host in a quantitative manner.

Keywords: DNA barcode-tagging; Next generation sequencing; Overlapping extension PCR; Population dynamics; Red recombination system; Salmonella.

MeSH terms

DNA Barcoding, Taxonomic / methods*
DNA, Bacterial / genetics*
High-Throughput Nucleotide Sequencing / methods
Humans
Polymerase Chain Reaction / methods
Salmonella / classification
Salmonella / genetics*
Salmonella Infections / microbiology*

Substances

DNA, Bacterial