Protocols of Conjugative Plasmid Transfer in Salmonella: Plate, Broth, and Filter Mating Approaches

Bijay K Khajanchi; Pravin R Kaldhone; Steven L Foley

doi:10.1007/978-1-4939-9570-7_12

Protocols of Conjugative Plasmid Transfer in Salmonella: Plate, Broth, and Filter Mating Approaches

Methods Mol Biol. 2019:2016:129-139. doi: 10.1007/978-1-4939-9570-7_12.

Authors

Bijay K Khajanchi¹, Pravin R Kaldhone², Steven L Foley²

Affiliations

¹ U.S. Food and Drug Administration, National Center for Toxicological Research, Jefferson, AR, USA. Bijay.Khajanchi@fda.hhs.gov.
² U.S. Food and Drug Administration, National Center for Toxicological Research, Jefferson, AR, USA.

PMID: 31197715
DOI: 10.1007/978-1-4939-9570-7_12

Abstract

Bacterial conjugation is a natural process that allows for horizontal transmission of DNA from one bacterium to another. Several plasmids carry transposons that encode multiple antimicrobial and metal resistance genes. Conjugative plasmid transfer requires intimate cell-to-cell contacts between the donor and the recipient. Self-conjugative plasmids harbor tra genes which facilitate plasmid transfer from donor to recipient bacterial strain. Here we describe different methods of conjugative plasmid transfers via conjugation.

Keywords: Conjugation; Plasmid; Salmonella; Transposons; tra genes.

MeSH terms

Conjugation, Genetic*
DNA Transposable Elements
Gene Transfer Techniques*
Gene Transfer, Horizontal
Humans
Plasmids / genetics*
Salmonella / genetics*
Salmonella Infections / microbiology

Substances

DNA Transposable Elements