Carbaryl waste-water treatment by Rhodopseudomonas sphaeroides

Abstract

Carbaryl wastewater treatment and the resource recycling of biomass as sludge by Rhodopseudomonas sphaeroides (R. sphaeroides) with the assistance of starch processing wastewater (SPW) was investigated in this research. It was observed that carbaryl was not degraded under the 100, 500 mg/L COD groups. The addition of SPW assisted R. sphaeroides to degrade carbaryl efficiently. Carbaryl removal reached 100% after 5 days under the optimal group (3500 mg/L). Interestingly, carbaryl in the mixed wastewater began to be degraded after day 1. Further research indicated that cehA gene was expressed after day 1. Subsequently, carbaryl hydrolase was synthesized under gene regulation. Analysis revealed that cehA and carbaryl hydrolase were adaptive gene expressions and enzymes. Carbaryl as stimulus signal started cehA gene expression through signal transduction pathway. This process took one day for R. sphaeroides. However, organics in 100, 500 mg/L COD groups were deficient, which could not maintain R. sphaeroides growth for over one day. Organics in SPW provided sufficient carbon sources for R. sphaeroides under other groups. The method could complete the mixed (SPW and carbaryl) wastewater treatment, carbaryl removal, the resource recycling of R. sphaeroides biomass as sludge simultaneously.

Keywords: Carbaryl hydrolase; Carbaryl wastewater; R. sphaeroides; SPW; cehA gene.