Long non-coding RNAs mortal obligate RNA transcript regulates the proliferation of human periodontal ligament stem cells and affects the recurrence of periodontitis

Yanhua Wang; Yingying Sun; Peng Zheng; Chunyu Cai; Yu Jiang; Huiyan Zhang; Zuntai Li; Qing Cai

doi:10.1016/j.archoralbio.2019.04.013

Long non-coding RNAs mortal obligate RNA transcript regulates the proliferation of human periodontal ligament stem cells and affects the recurrence of periodontitis

Arch Oral Biol. 2019 Sep:105:1-4. doi: 10.1016/j.archoralbio.2019.04.013. Epub 2019 Apr 27.

Authors

Yanhua Wang¹, Yingying Sun², Peng Zheng³, Chunyu Cai², Yu Jiang², Huiyan Zhang⁴, Zuntai Li⁴, Qing Cai⁵

Affiliations

¹ Department of Stomatology, Beijing Friendship Hospital, Capital Medical University, Beijing, 100050, PR China.
² Department of Stomatology, The First Hospital of Jilin University, Changchun City, Jilin Province, 130021, PR China.
³ Department of Endodontics, School and Hospital of Stomatology, Jilin University, Changchun City, Jilin Province, 130021, PR China.
⁴ Department of Dental Implantology, School and Hospital of Stomatology, Jilin University, Jinlin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Changchun City, Jilin Province, 130021, PR China.
⁵ Department of Dental Implantology, School and Hospital of Stomatology, Jilin University, Jinlin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Changchun City, Jilin Province, 130021, PR China. Electronic address: v630693@163.com.

PMID: 31195182
DOI: 10.1016/j.archoralbio.2019.04.013

Abstract

Objective: The aim of this study is to investigate the role of long non-coding RNAs (lncRNA), mortal obligate RNA transcript (MORT), in human periodontal ligament stem cells.

Design: Periodontal ligament tissues were collected from 48 periodontitis patients underwent tooth extraction and 38 people in similar age and gender distributions who experienced orthodontic treatment. After treatment, periodontitis patients were followed up for 2 years. MORT in periodontal ligament stem cells (PDLSCs) was detected by qRT-PCR. Proliferation of PDLSCs was detected by CCK-8 assay.

Results: The proliferation rate of PDLSCs isolated from periodontitis-affected teeth was significantly higher than PDLSCs from healthy teeth. Overexpression of MORT inhibited the proliferation of both types of periodontal ligament stem cells. After treatment, periodontitis patients were followed up for 2 years and patients with high level of MORT expression showed relatively lower recurrence rate comparing to low expression group.

Conclusion: MORT is involved in the proliferation of human PDLSCs and affects the recurrence of periodontitis.

Keywords: Periodontal ligament stem cells; Periodontitis; Proliferation; lncRNA MORT.

MeSH terms

Cell Differentiation
Cell Proliferation*
Cells, Cultured
Humans
Periodontal Ligament / cytology*
Periodontitis / genetics*
RNA, Long Noncoding / genetics*
Recurrence
Stem Cells / cytology*

Substances

MORT long noncoding RNA, human
RNA, Long Noncoding