Human monoclonal antibody: 2. Simultaneous expression of IgG and IgM with similar binding specificities by a human hybrid clone

Abstract

The human hybridoma clone JDB1 has been in continuous culture for over 40 months. This clone simultaneously produces both IgG and IgM. These two molecules have been separated and examined immunochemically. Both of the simultaneously secreted immunoglobulins contain lambda light chains. In addition, both of the antibodies react with breast cancer cells but not in an identical fashion. The implications of this multiple antibody response and the heterogeneity of tumor antigens is discussed.