Transcriptome Modifications in Porcine Adipocytes via Toll-Like Receptors Activation

Front Immunol. 2019 May 29:10:1180. doi: 10.3389/fimmu.2019.01180. eCollection 2019.

Abstract

Adipocytes are the most important cell type in adipose tissue playing key roles in immunometabolism. We previously reported that nine members of the Toll-like receptor (TLR) family are expressed in an originally established porcine intramuscular pre-adipocyte (PPI) cell line. However, the ability of TLR ligands to modulate immunometabolic transcriptome modifications in porcine adipocytes has not been elucidated. Herein, we characterized the global transcriptome modifications in porcine intramuscular mature adipocytes (pMA), differentiated from PPI, following stimulation with Pam3csk4, Poly(I:C) or LPS which are ligands for TLR2, TLR3, and TLR4, respectively. Analysis of microarray data identified 530 (218 up, 312 down), 520 (245 up, 275 down), and 525 (239 up, 286 down) differentially expressed genes (DEGs) in pMA following the stimulation with Pam3csk4, Poly(I:C), and LPS, respectively. Gene ontology classification revealed that DEGs are involved in several biological processes including those belonging to immune response and lipid metabolism pathways. Functionally annotated genes were organized into two groups for downstream analysis: immune response related genes (cytokines, chemokines, complement factors, adhesion molecules, and signal transduction), and genes involved with metabolic and endocrine functions (hormones and receptors, growth factors, and lipid biosynthesis). Differential expression analysis revealed that EGR1, NOTCH1, NOS2, TNFAIP3, TRAF3IP1, INSR, CXCR4, PPARA, MAPK10, and C3 are the top 10 commonly altered genes of TLRs induced transcriptional modification of pMA. However, the protein-protein interaction network of DEGs identified EPOR, C3, STAR, CCL2, and SAA2 as the major hub genes, which were also exhibited higher centrality estimates in the Gene-Transcription factor interaction network. Our results provide new insights of transcriptome modifications associated with TLRs activation in porcine adipocytes and identified key regulatory genes that could be used as biomarkers for the evaluation of treatments having immunomodularoty and/or metabolic functional beneficial effects in porcine adipocytes.

Keywords: TLRs (Toll-like receptors); adipocytes; immunometabolism; microarray; pig; transcriptome.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipocytes / metabolism*
  • Adipogenesis / drug effects*
  • Adipogenesis / genetics
  • Adipose Tissue / metabolism*
  • Animals
  • Cell Line
  • Female
  • Gene Expression Regulation / drug effects*
  • Gene Ontology
  • Lipopeptides / pharmacology
  • Lipopolysaccharides / pharmacology
  • Metabolic Networks and Pathways / genetics
  • Muscle, Skeletal / cytology
  • Oligonucleotide Array Sequence Analysis
  • Poly I-C / pharmacology
  • RNA, Messenger / biosynthesis
  • Signal Transduction / drug effects
  • Swine
  • Toll-Like Receptor 2 / drug effects*
  • Toll-Like Receptor 2 / physiology
  • Toll-Like Receptor 3 / drug effects*
  • Toll-Like Receptor 3 / physiology
  • Toll-Like Receptor 4 / drug effects*
  • Toll-Like Receptor 4 / physiology
  • Transcriptome / drug effects*

Substances

  • Lipopeptides
  • Lipopolysaccharides
  • Pam(3)CSK(4) peptide
  • RNA, Messenger
  • Toll-Like Receptor 2
  • Toll-Like Receptor 3
  • Toll-Like Receptor 4
  • Poly I-C