Differential Regulation of Stomatal Conductance as a Strategy to Cope With Ammonium Fertilizer Under Ambient Versus Elevated CO2

Fernando Torralbo; María Begoña González-Moro; Edurne Baroja-Fernández; Iker Aranjuelo; Carmen González-Murua

doi:10.3389/fpls.2019.00597

Differential Regulation of Stomatal Conductance as a Strategy to Cope With Ammonium Fertilizer Under Ambient Versus Elevated CO₂

Front Plant Sci. 2019 May 24:10:597. doi: 10.3389/fpls.2019.00597. eCollection 2019.

Authors

Fernando Torralbo¹, María Begoña González-Moro¹, Edurne Baroja-Fernández², Iker Aranjuelo², Carmen González-Murua¹

Affiliations

¹ Department of Plant Biology and Ecology, University of the Basque Country (UPV/EHU), Bilbao, Spain.
² Instituto de Agrobiotecnología (IdAB)-CSIC, Mutilva, Spain.

Abstract

While nitrogen (N) derived from ammonium would be energetically less expensive than nitrate-derived N, the use of ammonium-based fertilizer is limited by the potential for toxicity symptoms. Nevertheless, previous studies have shown that exposure to elevated CO₂ favors ammonium assimilation in plants. However, little is known about the impact of different forms of N fertilizer on stomatal opening and their consequent effects on CO₂ and H₂O diffusion in wheat plants exposed to ambient and elevated CO₂. In this article, we have examined the response of the photosynthetic machinery of durum wheat (Triticum durum, var. Amilcar) grown with different types of N fertilizer (NO₃ ^-, NH₄ ⁺, and NH₄NO₃) at 400 versus 700 ppm of CO₂. Alongside gas exchange and photochemical parameters, the expression of genes involved in CO₂ (PIP1.1 and PIP2.3) and H₂O (TIP1) diffusion as well as key C and N primary metabolism enzymes and metabolites were studied. Our results show that at 400 ppm CO₂, wheat plants fertilized with ammonium as the N source had stress symptoms and a strong reduction in stomatal conductance, which negatively affected photosynthetic rates. The higher levels of PIP1.1 and PIP2.3 expression in ammonium-fertilized plants at 400 ppm CO₂ might reflect the need to overcome limitations to the CO₂ supply to chloroplasts due to restrictions in stomatal conductance. This stomatal limitation might be associated with a strategy to reduce ammonium transport toward leaves. On the other hand, ammonium-fertilized plants at elevated CO₂ did not show stress symptoms, and no differences were detected in stomatal opening or water use efficiency (WUE). Moreover, similar gene expression of the aquaporins TIP1, PIP1.1, and PIP2.3 in ammonium-fertilized plants grown at 700 ppm compared to nitrate and ammonium nitrate plants would suggest that an adjustment in CO₂ and H₂O diffusion is not required. Therefore, in the absence of a stress context triggered by elevated CO₂, ammonium- and ammonium nitrate-fertilized plants were able to increase their photosynthetic rates, which were translated eventually into higher leaf protein content.

Keywords: N assimilation; ammonium; aquaporins; elevated CO2; nitrate; stomata conductance; wheat.