Objective: To develop a method for determining diphenylmethane diisocyanate in workplace air by HPLC with impregnated filter membrane. Methods: MDI in workplace air reacted with 1- (2-pyridyl) piperazine on impregnated filter membrane to form MDI-urea derivatives, after elution and filtration, it was detected by HPLC-UV. Results: Limit of detection was 0.003 8 μg/ml and limit of quantification was 0.013 μg/ml. Good linearity was obtained in the range of 0.013~2.000 μg/ml (r=0.999 7) . The precision was 3.10%~8.03% (n=6) , while the recovery was 96.3%~101.9%. Asorption capacity of the membrane was 40.8 μg MDI, and could be stored for 14 days in the light-proof environment of 2~8 ℃. Conclusion: The method optimized testing steps for MDI's standard curve, and provided good guidance for determination of MDI in workplace air with impregnated fiter membrane.
目的： 建立工作场所空气中二苯基甲烷二异氰酸酯（MDI）的浸渍滤纸采集-高效液相色谱测定方法。 方法： 空气中二苯基甲烷二异氰酸酯与浸渍滤纸上的1-（2-吡啶基）哌嗪反应生成MDI-脲衍生物而被吸附于滤纸上，经洗脱、过滤后，高效液相色谱仪测定。 结果： 二苯基甲烷二异氰酸酯的检出限为0.003 8 μg/ml，定量限为0.013 μg/ml，在0.013~2.000 μg/ml范围内线性良好（r=0.999 7），方法精密度为3.10%~8.03%（n=6），回收率为96.3%~101.9%，滤纸吸附容量为40.8 μg MDI，样品在2 ℃~8 ℃避光环境中可保存14 d。 结论： 本方法优化MDI标准曲线的配制方法，建立了测定工作场所空气中MDI的浸渍滤纸采集-高效液相色谱法。.
Keywords: Diphenylmethane diisocyanate; HPLC; Impregnated filter membrane.