Abstract
We performed a comparative study of the cytotoxic effect of endocannabinoid N-arachidonoyl dopamine (AA-DA) on cultured stromal cells of ectopic and eutopic endometrium. It was found that AA-DA in the concentration range of 1-20 μM produces more selective cytotoxic effect on the stromal cells of the ectopic endometrium due to interaction with cannabinoid type 1 receptor. In concentrations below 1 μM, AA-DA stimulated the proliferation of stromal cells of the eutopic endometrium and did not affect the division of ectopic endometrium cells. This effect was realized due to its interaction with cannabinoid type 2 receptor.
Keywords:
N-arachidonoyl dopamine; cytotoxicity; endometriosis; proliferation; stromal cells.
MeSH terms
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Camphanes / pharmacology
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Cannabinoid Receptor Antagonists / pharmacology
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Capsaicin / analogs & derivatives
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Capsaicin / pharmacology
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Cell Proliferation / drug effects*
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Cell Survival / drug effects*
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Dopamine / metabolism*
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Endometriosis / metabolism*
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Endometrium / cytology
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Endometrium / metabolism*
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Female
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Humans
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Pyrazoles / pharmacology
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Receptor, Cannabinoid, CB1 / antagonists & inhibitors
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Receptor, Cannabinoid, CB1 / metabolism
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Receptor, Cannabinoid, CB2 / antagonists & inhibitors
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Receptor, Cannabinoid, CB2 / metabolism
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Rimonabant / pharmacology
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Stromal Cells / cytology*
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Stromal Cells / drug effects*
Substances
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Camphanes
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Cannabinoid Receptor Antagonists
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Pyrazoles
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Receptor, Cannabinoid, CB1
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Receptor, Cannabinoid, CB2
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SR 144528
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capsazepine
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Rimonabant
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Capsaicin
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Dopamine