Full-factorial central composite rotational design for the immobilization of lactase in natural polysaccharide-based hydrogels and hydrolysis of lactose

Abstract

A full-factorial central composite rotational design (FFCCRD) was applied for studying the immobilization of lactase in Arabic gum-based and chitosan-based hydrogels, and hydrolysis of lactose. The optimal immobilization capacities of both hydrogels aiming to obtain high immobilized enzyme activity and low released fraction were determined at 25.0 °C, 39.88 mg mL^-1 initial enzyme concentration and pH 6.5. The immobilized enzyme activity and released fraction from the Arabic gum-based hydrogel were 0.322 U mg^-1 and 0.193, respectively, during the hydrolysis of lactose contained in UHT milk. These values were 0.289 U mg^-1 and 0.136, respectively, using a chitosan-based hydrogel. The immobilized enzyme activity and released fraction from these hydrogels during the hydrolysis of standard lactose were 0.246 U mg^-1 and 0.407, and 0.211 U mg^-1 and 0.245, respectively. The best conditions for the immobilization of lactase and hydrolysis of lactose were achieved by applying FFCCRD, which were compared with experimental results.

Keywords: Factorial design; Hydrogel; Hydrolysis; Lactase; Lactose.