TLR2, Siglec-3 and CD163 expressions on porcine peripheral blood monocytes are increased during sepsis caused by Haemophilus parasuis

Álvaro Álvarez-Estrada; Elías Fernando Rodríguez-Ferri; Sonia Martínez-Martínez; Belén Álvarez; Teresa Fernández-Caballero; Javier Domínguez; Cesar Bernardo Gutiérrez-Martín

doi:10.1016/j.cimid.2019.02.001

TLR2, Siglec-3 and CD163 expressions on porcine peripheral blood monocytes are increased during sepsis caused by Haemophilus parasuis

Comp Immunol Microbiol Infect Dis. 2019 Jun:64:31-39. doi: 10.1016/j.cimid.2019.02.001. Epub 2019 Feb 8.

Authors

Álvaro Álvarez-Estrada¹, Elías Fernando Rodríguez-Ferri², Sonia Martínez-Martínez³, Belén Álvarez⁴, Teresa Fernández-Caballero⁵, Javier Domínguez⁶, Cesar Bernardo Gutiérrez-Martín⁷

Affiliations

¹ Microbiology & Immunology Section, Animal Health Department, Faculty of Veterinary Medicine, University of León, Spain. Electronic address: aalve@unileon.es.
² Microbiology & Immunology Section, Animal Health Department, Faculty of Veterinary Medicine, University of León, Spain. Electronic address: ef.rferri@unileon.es.
³ Microbiology & Immunology Section, Animal Health Department, Faculty of Veterinary Medicine, University of León, Spain. Electronic address: smarm@unileon.es.
⁴ Porcine Immunology Laboratory, Biotechnology Department, INIA, Madrid, Spain. Electronic address: avega@inia.es.
⁵ Porcine Immunology Laboratory, Biotechnology Department, INIA, Madrid, Spain. Electronic address: fernandez.teresa@inia.es.
⁶ Porcine Immunology Laboratory, Biotechnology Department, INIA, Madrid, Spain. Electronic address: juncal@inia.es.
⁷ Microbiology & Immunology Section, Animal Health Department, Faculty of Veterinary Medicine, University of León, Spain. Electronic address: cbgutm@unileon.es.

PMID: 31174697
DOI: 10.1016/j.cimid.2019.02.001

Abstract

TLRs, Siglecs and CD163 are cell surface receptors that play an important role in immune response and sepsis. The objective of this study was to assess changes in the expression levels of several of these receptors (TLR2, TLR4, CD163, Siglec-1, Siglec-3, Siglec-5 and Siglec-10) on the surface of peripheral blood mononuclear cells from pigs with sepsis caused by Haemophilus parasuis. Flow cytometry was employed to analyze samples from an experimental infection and from cell cultures. A significant increase in CD163, TLR2 and Siglec-3 expression during infection was seen. However, in vitro exposure of peripheral blood monocytes to bacteria or sera from infected pigs did not increase the expression of these receptors. These changes may be due to recruitment of monocytes into the blood compartment in response to H. parasuis-induced sepsis.

Keywords: CD163; Glässer´s disease; Haemophilus parasuis; Sepsis; Siglec; TLR.

MeSH terms

Animals
Antigens, CD / genetics*
Antigens, CD / immunology
Antigens, Differentiation, Myelomonocytic / genetics*
Antigens, Differentiation, Myelomonocytic / immunology
Cells, Cultured
Haemophilus Infections / immunology
Haemophilus Infections / veterinary*
Haemophilus parasuis
Monocytes / immunology*
Monocytes / microbiology
Receptors, Cell Surface / genetics*
Receptors, Cell Surface / immunology
Sepsis / immunology
Sepsis / microbiology
Sepsis / veterinary*
Sialic Acid Binding Ig-like Lectin 3 / genetics*
Sialic Acid Binding Ig-like Lectin 3 / immunology
Swine
Swine Diseases / immunology*
Swine Diseases / microbiology
Toll-Like Receptor 2 / genetics*
Toll-Like Receptor 2 / immunology

Substances

Antigens, CD
Antigens, Differentiation, Myelomonocytic
CD163 antigen
Receptors, Cell Surface
Sialic Acid Binding Ig-like Lectin 3
Toll-Like Receptor 2