Rational Design and Delivery of NOX-Inhibitory Peptides

Methods Mol Biol. 2019:1982:417-428. doi: 10.1007/978-1-4939-9424-3_24.

Abstract

A growing appreciation of NADPH oxidases (NOXs) as mediators of fundamental physiological processes and as important players in myriad diseases has led many laboratories on a search for specific inhibitors to help dissect the role in a given pathway or pathological condition. To date, there are only a few available inhibitors with a demonstrated specificity for a given isozyme. Among those, peptidic inhibitors have the advantage of being designed to target very specific protein-protein interactions that are essential for NOX activity. Herein, we provide the techniques to deliver these inhibitors both in cell culture as well as in vivo.

Keywords: Isoform-specific inhibitors; NADPH oxidase; Nox2ds-tat; NoxA1ds; Peptidic inhibitors.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Carrier Proteins
  • Cell Line
  • Cells, Cultured
  • Drug Delivery Systems
  • Drug Design*
  • Enzyme Inhibitors / administration & dosage
  • Enzyme Inhibitors / chemistry*
  • Enzyme Inhibitors / pharmacology*
  • Humans
  • Models, Biological
  • NADPH Oxidases / antagonists & inhibitors*
  • NADPH Oxidases / chemistry*
  • Peptides / administration & dosage
  • Peptides / chemistry*
  • Peptides / pharmacology*
  • Protein Binding
  • Protein Interaction Mapping
  • Reactive Oxygen Species / metabolism

Substances

  • Carrier Proteins
  • Enzyme Inhibitors
  • Peptides
  • Reactive Oxygen Species
  • NADPH Oxidases