Quantitative Imaging of Endogenous and Exogenous H2O2 Gradients in Live Zebrafish Larvae

Methods Mol Biol. 2019:1982:283-299. doi: 10.1007/978-1-4939-9424-3_17.

Abstract

Quantitative aspects of extracellular H2O2 signaling in animals, such as its spatiotemporal dynamics within tissues, remain little understood. Here we detail an optimized, experimental setup for measuring the dynamics and physiological consequences of extracellular H2O2 application to live tissues by intravital biosensor imaging in zebrafish larvae.

Keywords: Biosensor; Diffusion; Gradient; Hydrogen peroxide; Intravital; Quantitative; Zebrafish.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biosensing Techniques
  • Hydrogen Peroxide / metabolism*
  • Image Processing, Computer-Assisted
  • Larva
  • Molecular Imaging* / methods
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Signal Transduction
  • Zebrafish / metabolism*
  • Zebrafish Proteins / genetics
  • Zebrafish Proteins / metabolism*

Substances

  • RNA, Messenger
  • Zebrafish Proteins
  • Hydrogen Peroxide