Use of an electro-optical sensor and phage antibodies for immunodetection of Herbaspirillum

Abstract

A sheep single-chain antibody-fragment library (Griffin.1, UK) was used to obtain miniantibodies to the lipopolysaccharide of Herbaspirillum seropedicae Z78. Using electro-optical analysis and electron microscopy, we recorded a biospecific interaction of antigenic determinants on the cell surface with phage antibodies against the LPS of H. seropedicae Z78 (mini-Abs_LPS). Control experiments were run to rule out nonspecific binding of the mini-Abs_LPS to cells of Azospirillum brasilense Sp245. Use of the highly specific mini-Abs_LPS enabled the lipopolysaccharide of H. seropedicae Z78 to be detected in a mixture of bacterial cells by electro-optical means (analysis time, ∼5 min). This report is the first to show the possibility of rapid detection of Herbaspirillum on the basis of electro-optical analysis coupled with the use of mini-Abs_LPS. The results are promising for the development of biosensor-based methods to detect potentially human-harmful prokaryotes whose structures either have not been studied or are absent from commercial databases.

Keywords: Detection; Electro-optical sensor; Herbaspirillum; Lipopolysaccharide; Phage antibodies.