Label-free Pb2+ detection on the layer-by-layer platform using real-time dual polarization interferometry

Shuang Wang; Shasha Lu; Jiahui Zhao; Jianshe Huang; Xiurong Yang

doi:10.1016/j.talanta.2019.05.015

Label-free Pb²⁺ detection on the layer-by-layer platform using real-time dual polarization interferometry

Talanta. 2019 Sep 1:202:336-341. doi: 10.1016/j.talanta.2019.05.015. Epub 2019 May 3.

Authors

Shuang Wang¹, Shasha Lu¹, Jiahui Zhao², Jianshe Huang³, Xiurong Yang⁴

Affiliations

¹ State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Changchun, Jilin, 130022, China; University of Science and Technology of China, Hefei, Anhui, 230026, China.
² State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Changchun, Jilin, 130022, China; University of Chinese Academy of Sciences, Beijing, 100039, China.
³ State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Changchun, Jilin, 130022, China.
⁴ State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Changchun, Jilin, 130022, China; University of Science and Technology of China, Hefei, Anhui, 230026, China; University of Chinese Academy of Sciences, Beijing, 100039, China. Electronic address: xryang@ciac.ac.cn.

PMID: 31171191
DOI: 10.1016/j.talanta.2019.05.015

Abstract

Contamination of Lead ion (Pb²⁺) poses a serious threat to human health and the environment. Developing sensitive and reliable Pb²⁺ biosensor is essential and significant for prevention and control of the water pollution. In this work, we utilized the direct conformational signal changes in specific interaction process between Pb²⁺ and guanine-rich (G-rich) oligonucleotides to establish a sensing system. The binding of Pb²⁺ and G-rich oligonucleotides results in conformational transition from single strand to G-quadruplex, which was recorded real-time by dual polarization interferometry (DPI). The sensing platform was established via layer-by-layer assembly strategy that employing polyethyleneimine and ploythymine as media layers to link the G-rich DNA on the DPI chip, which is simple, facile and without need of synthesizing sophisticated nanomaterial. On the basis of above sensing platform and quantifiable conformational changes, a simple, sensitive, specific and label-free Pb²⁺ biosensor was established. This research provides us a simple and sensitive method for detection of ions, also encourages us to combine surface technique to biosensing application and explore the relationship between structures and functions.

Keywords: Biosensor; Dual polarization interferometry; G-quadruplex; Layer-by-layer assembly; Lead ion.