Protein film electrochemical EPR spectroscopy as a technique to investigate redox reactions in biomolecules

Kaltum Abdiaziz; Enrico Salvadori; Katarzyna P Sokol; Erwin Reisner; Maxie M Roessler

doi:10.1039/c9cc03212f

Protein film electrochemical EPR spectroscopy as a technique to investigate redox reactions in biomolecules

Chem Commun (Camb). 2019 Jul 23;55(60):8840-8843. doi: 10.1039/c9cc03212f.

Authors

Kaltum Abdiaziz¹, Enrico Salvadori, Katarzyna P Sokol, Erwin Reisner, Maxie M Roessler

Affiliation

¹ School of Biological and Chemical Sciences and Materials Research Institute, Queen Mary University of London, Mile End Road, London E1 4NS, UK.

PMID: 31168558
DOI: 10.1039/c9cc03212f

Abstract

Redox reactions and paramagnetic intermediates are ubiquitous in biological chemistry. We report a new method, protein film electrochemical electron paramagnetic resonance spectroscopy (PFE-EPR), that enables the direct and accurate potential control of proteins on the electrode surface for both electrochemical and EPR spectroscopic characterisation of their redox centres.

MeSH terms

Animals
Cattle
Cyclic N-Oxides / chemistry*
Electrochemical Techniques / instrumentation
Electrochemical Techniques / methods*
Electrodes
Electron Spin Resonance Spectroscopy / methods*
Oxidation-Reduction
Superoxide Dismutase-1 / chemistry*

Substances

Cyclic N-Oxides
4-amino-TEMPO
Superoxide Dismutase-1