Identification of differentially expressed transcripts at critical developmental stages in sorghum [ Sorghum bicolor (L.) Moench] in relation to grain yield heterosis

I Jaikishan; P Rajendrakumar; K Hariprasanna; D Balakrishna; B Venkatesh Bhat; Vilas A Tonapi

doi:10.1007/s13205-019-1777-6

Identification of differentially expressed transcripts at critical developmental stages in sorghum [ Sorghum bicolor (L.) Moench] in relation to grain yield heterosis

3 Biotech. 2019 Jun;9(6):239. doi: 10.1007/s13205-019-1777-6. Epub 2019 May 29.

Authors

I Jaikishan¹, P Rajendrakumar¹, K Hariprasanna¹, D Balakrishna¹, B Venkatesh Bhat¹, Vilas A Tonapi¹

Affiliation

¹ ICAR-Indian Institute of Millets Research, Hyderabad, Telangana 500030 India.

Abstract

Evaluation of a set of 10 F₁ hybrids along with their female (27A and 7A) and male parents (C 43, RS 673, RS 627, CB 26, and CB 29) for grain yield and its component traits revealed that grain yield/plant followed by panicle weight, primary branches/panicle, and 100-seed weight exhibited high levels of heterosis. Eight hybrids exhibited 50% or more mid-parent heterosis for grain yield/plant, of which, one hybrid (27A × RS673) recorded heterobeltiosis above 50% (73.61%). Differential display analysis generated about 2995 reproducible transcripts, which were categorized as UPF₁-expressed in any one of the parents and F₁ (10.53-14.76%), BPnF₁-expressed in both parents but not in F₁ (4.56-11.44%), UPnF₁-expressed in either of the parents and not in F₁ (17.95-27.40%), F₁nBP-expressed only in F₁ but not in either of the parents (14.39-20.54%), and UET-expressed in both parents and F₁ (34.52-42.43%). A comparison between high and low heterotic hybrids revealed that the proportions of UPF₁ and F₁nBP transcript patterns were much higher in the former (21.31% and 45.24%) as compared to the latter (16.67% and 32.14%) at the booting and flowering stage, respectively, indicating the role of over-dominance and dominance in the manifestation of grain yield heterosis. Significant positive correlations were observed for differential transcript patterns with mid-parent and better-parent heterosis for the components of grain yield such as primary branches (0.63 and 0.61 at p < 0.01) and 100-seed weight (0.64 and 0.52 at p < 0.01). Cloning and sequence analysis of 16 transcripts that were differentially expressed in hybrids and their parental lines revealed that they code for genes involved in basic cellular processes, cellulose biosynthesis, and assimilate partitioning between various organs and allocation between various pathways, pyrimidine, and polyamine biosynthesis, enhancing ATP production and regulation of plant growth and development.

Keywords: Correlation; Differential display analysis; Differential expression; Grain yield heterosis; Sorghum; Transcripts.