Enzymatic synthesis of N-acetyllactosamine from lactose enabled by recombinant β1,4-galactosyltransferases

Org Biomol Chem. 2019 Jun 18;17(24):5920-5924. doi: 10.1039/c9ob01089k.

Abstract

Utilising a fast and sensitive screening method based on imidazolium-tagged probes, we report unprecedented reversible activity of bacterial β1,4-galactosyltransferases to catalyse the transgalactosylation from lactose to N-acetylglucosamine to form N-acetyllactosamine in the presence of UDP. The process is demonstrated by the preparative scale synthesis of pNP-β-LacNAc from lactose using β1,4-galactosyltransferase NmLgtB-B as the only biocatalyst.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Sugars / biosynthesis*
  • Amino Sugars / chemistry
  • Biocatalysis
  • Galactosyltransferases / chemistry
  • Galactosyltransferases / metabolism*
  • Lactose / chemistry
  • Lactose / metabolism*
  • Molecular Structure
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / metabolism

Substances

  • Amino Sugars
  • Recombinant Proteins
  • N-acetyllactosamine
  • Galactosyltransferases
  • Lactose