Use of Rhizobacteria and Mycorrhizae Consortium in the Open Field as a Strategy for Improving Crop Nutrition, Productivity and Soil Fertility

Anas Raklami; Noura Bechtaoui; Abdel-Ilah Tahiri; Mohamed Anli; Abdelilah Meddich; Khalid Oufdou

doi:10.3389/fmicb.2019.01106

Use of Rhizobacteria and Mycorrhizae Consortium in the Open Field as a Strategy for Improving Crop Nutrition, Productivity and Soil Fertility

Front Microbiol. 2019 May 21:10:1106. doi: 10.3389/fmicb.2019.01106. eCollection 2019.

Authors

Anas Raklami^{1

2}, Noura Bechtaoui¹, Abdel-Ilah Tahiri^{1

2}, Mohamed Anli², Abdelilah Meddich², Khalid Oufdou¹

Affiliations

¹ Laboratory of Biology and Biotechnology of Microorganisms, Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakesh, Morocco.
² Laboratory of Biotechnology and Plant Physiology, Faculty of Sciences Semlalia, Cadi Ayyad University, Marrakesh, Morocco.

Abstract

Plant growth promoting rhizobacteria (PGPR) and arbuscular mycorrhizal fungi (AMF) are known for their beneficial effects. In recent years, more attention has been paid to their use as biofertilizers to reduce the use of chemical fertilizers causing significant damage to the environment. To have high plant yields, biofertilizers may not be able to sustain plant demands and could be used in combination with chemical fertilizers. However, the application of biofertilizers in the field such as rhizobacteria and AMF are understudied and powerfully needed. In this context, this study aims to evaluate the effect of inoculation with rhizobacteria and AMF and their potential to stimulate two of the most economically important crops in Mediterranean semi-arid areas (Vicia faba L. and Triticum durum L.). The effect of inoculation was studied in field experiment with six treatments: (i) the control without inoculation (C), (ii) PGPR alone (PG), (iii) rhizobia alone (R), (iv) the mixture of PGPR and rhizobia (PR), (v) AMF alone (M), and (vi) the mixture of PGPR, rhizobia and AMF (PRM). The inoculation with the consortium of PGPR-rhizobia-AMF (PRM) induced the greatest effect. This inoculation improved the growth parameters (dry weight of shoots and roots) of faba bean and wheat. An improvement of 130, 200, and 78% was observed in V. faba shoot and root dry weight, and the number of leaves, respectively. Similarly, shoot and root dry weight and number of leaves of T. durum were enhanced by 293, 258, and 87%, respectively. The inoculation improved the productivity of studied plants presented by the number and weight of bean pods (270 × 10⁴ ha^-1 and 30737.5 kg.ha^-1) and wheat spikes (440 × 10⁴ ha^-1 and 10560 kg.ha^-1). In addition, the mineral analyses showed that the inoculation with PGPR-rhizobia-mycorrhizae improved N, P, Ca, K, and Na shoots contents, as well as the contents of sugar and proteins. Finally, we revealed the positive impact of the tested biofertilizers and the interest of adoption of innovative practices improving crops productivity and soil fertility.

Keywords: PGPR; biofertilizers; mediterranean region; nutrient uptake; rhizosphere.