Herein we describe a methodology to synthesis polyurethane foam molecularly imprinted polymer (PUF-MIP) by using functional monomer for selective extraction of alprazolam. For this purpose, the various percentages of functional monomer are used to synthesis PUF-MIP of alprazolam. To evaluate the selectivity of synthesized PUF-MIP HPLC analysis is applied by introducing caffeine and methadone as an interference. To optimize the proposed technique, effective parameters in the SPE procedure including pH, flow, and salt present is investigated by experimental design. Finally, this method is evaluated in urine sample to monitor alprazolam dosage. In the optimized condition, the synthesized polymer indicates high selectivity value about 71% for alprazolam and 96.8% recovery for MIPUF compared with non-imprinted polyurethane foam (NIPUF). The linear dynamic range (LDR) of 0.03-60 mg L-1, the limit of detection of 8-10 μg L-1, the relative standard deviation (RSD, n = 3) of 2.88-3.65 % and quantification of 25-30 μg L-1 is obtained for HPLC analysis based on PUF-MIP extraction.
Keywords: Alprazolam; Functional monomer; High performance liquid chromatography; Molecularly imprinted polymer; Polyurethane foam.
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