Removal of nine pesticide residues from water and soil by biosorption coupled with degradation on biosorbent immobilized laccase

Abstract

Biosorbents prepared with peanut shell and wheat straw were act as supports for the immobilization of Aspergillus laccase, and the redox mediator syringaldehyde (SA) was used to improve laccase-catalyzed degradation of nine pesticide residues, including isoproturon, atrazine, prometryn, mefenacet, penoxsulam, nitenpyram, prochloraz, pyrazosulfuron-ethyl and bensulfuron-methyl. Pesticides in water and soil samples were effectively removed via biosorbent concentration and subsequent immobilized laccase degradation on peanut shell or wheat straw supports. The Langmuir equation and Freundlich equation described the biosorption isotherms of the nine pesticides. Parameters that affect the degradation was also investigated. With concentration of pesticides and SA of 6.0 mg L^-1 and 1 mmol L^-1, over 54.5% and 65.9% of pesticides were removed in water in 3 days with a biosorbent immobilized laccase dose of 25 g L^-1 for peanut shell immobilized laccase and wheat straw immobilized laccase, respectively. In the treatment of pesticide in soil with a biosorbent dose of 50 g (kg soil)^-1, with the maximum degradation rates ranged from 20.9 to 92.9% and 14.7-92.0% in 7 days for peanut shell immobilized laccase and wheat straw immobilized laccase, respectively. Therefore, laccase immobilized on biomass materials has a strong potential for the effective removal of pesticide pollutants from water and soil by biosorption coupled with degradation.

Keywords: Biomass material; Biosorption; Degradation; Immobilized laccase; Pesticide.