Thermodynamic Activity-Based Solvent Design for Bioreactions

Anton Wangler; Christoph Held; Gabriele Sadowski

doi:10.1016/j.tibtech.2019.04.015

Thermodynamic Activity-Based Solvent Design for Bioreactions

Trends Biotechnol. 2019 Oct;37(10):1038-1041. doi: 10.1016/j.tibtech.2019.04.015. Epub 2019 May 31.

Authors

Anton Wangler¹, Christoph Held², Gabriele Sadowski¹

Affiliations

¹ Laboratory of Thermodynamics, Department of Biochemical and Chemical Engineering, Technische Universität (TU) Dortmund, Emil-Figge-Strasse 70, 44227 Dortmund, Germany.
² Laboratory of Thermodynamics, Department of Biochemical and Chemical Engineering, Technische Universität (TU) Dortmund, Emil-Figge-Strasse 70, 44227 Dortmund, Germany. Electronic address: Christoph.Held@tu-dortmund.de.

PMID: 31160055
DOI: 10.1016/j.tibtech.2019.04.015

Abstract

To improve the kinetics of enzyme-catalyzed reactions, cosolvents are commonly added to reaction mixtures. The search for a good cosolvent is still empirical and experimentally based. We discuss a thermodynamic activity-based approach that improves biocatalytic processes by predicting cosolvent influences on Michaelis constants, ultimately reducing time and cost.

Keywords: Michaelis constant; activity coefficients; biocatalysis; cosolvent; ePC-SAFT; predictions.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alcohol Dehydrogenase / chemistry
Alcohol Dehydrogenase / metabolism
Biocatalysis*
Enzymes / chemistry*
Enzymes / metabolism*
Kinetics
Models, Theoretical
Solvents / chemistry*
Solvents / metabolism
Thermodynamics

Substances

Enzymes
Solvents
Alcohol Dehydrogenase