Abstract
In this study, we found that dipeptide transporter Ptr2p is the putative transporter of read-through compounds (+)-negamycin derivatives TCP-126 and TCP-112, in budding yeast. Ptr2p expression and activity were correlated with the TCP-112 sensitivity, and dipeptide with high affinity to Ptr2p suppressed the TCP-112 activity. These results suggest that dipeptide transporter is one of the determinants of negamycin analogs sensitivity. Abbreviation: PTC: premature termination codon.
Keywords:
Dipeptide transporter; Ubr1p-Cup9p-Ptr2p pathway; negamycin; readthrough.
MeSH terms
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Amino Acids, Diamino / metabolism
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Biological Transport
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Genes, Fungal
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Homeodomain Proteins / genetics
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Homeodomain Proteins / metabolism*
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Membrane Transport Proteins / genetics
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Membrane Transport Proteins / metabolism*
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / metabolism*
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Saccharomyces cerevisiae Proteins / genetics
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Saccharomyces cerevisiae Proteins / metabolism*
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Ubiquitin-Protein Ligases / genetics
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Ubiquitin-Protein Ligases / metabolism*
Substances
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Amino Acids, Diamino
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CUP9 protein, S cerevisiae
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Homeodomain Proteins
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Membrane Transport Proteins
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PTR2 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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negamycin
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UBR1 protein, S cerevisiae
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Ubiquitin-Protein Ligases