Application of a microalga, Scenedesmus obliquus PF3, for the biological removal of nitric oxide (NO) and carbon dioxide

Abstract

Nitrogen oxide (NOx) emissions from flue gas lead to a series of environmental problems. Biological removal of Nitrogen oxide (NOx) from flue gas by microalgae is a potential approach for reducing the problems caused by these emissions. However, few microalgal strains are reported to remove NOx from flue gas. Here, a microalga strain PF3 (identified as Scenedesmus obliquus), which can remove NOx and fix CO₂ from flue gas is isolated. The tolerance of Scenedesmus obliquus PF3 to CO₂, NO, SO₂ and its adaptabilities to environmental factors (pH and temperature), and its performance in the removal of NO and CO₂ are investigated. Scenedesmus obliquus PF3 showed biomass accumulation when sparged with 15% CO₂ or 500 ppm NO or 50 ppm SO₂, and bisulfite less than 2 mM showed no toxicity to Scenedesmus obliquus PF3. Additionally, PF3 grew well in a wide range of pH and temperatures from 4.5 to 10.5 and 15 °C-30 °C, respectively. When sparged with simulated flue gas (100 ppm NO, 10% CO₂, (N₂ as balance gas)), the microalgae culture system removed NO and CO₂ at a rate of 2.86 ± 0.23 mg L^-1 d^-1 and 1.48 ± 0.12 g L^-1 d^-1, respectively, where up to 96.9 ± 0.03% (2.77 ± 0.08 mg L^-1 d^-1) and 87.7 ± 6.22% (1.29 ± 0.01 mg L^-1 d^-1) of the removed NO and CO₂, respectively, were assimilated in algal biomass. These results suggest that Scenedesmus obliquus PF3 is a promising candidate for NOx removal and carbon fixation of flue gas.

Keywords: CO(2) fixation; DeNOx; Flue gas; Microalgae isolation; Scenedesmus obliquus.