Anxiolytic- and anxiogenic-like effects of Montanoa tomentosa (Asteraceae): Dependence on the endocrine condition

Abstract

Ethnopharmacological relevance: Montanoa tomentosa Cerv. (MT) is a native plant from Mexico used in traditional medicine as a remedy for reproductive impairments and relaxing effects. In previous studies, it has been shown that the endocrine state could modify the antianxiety-like actions of anxiolytic compounds. Although women are the primary user of MT, no studies have evaluated the potential impact of the endocrine milieu on its anti-anxiety actions.

Aims of the study: Ascertain the antianxiety effects of M. tomentosa in rats with different hormonal conditions, and to analyze the participation of the GABA_A receptor in ovariectomized rats treated with MT.

Materials and methods: The animal model of anxiety used was the elevated plus-maze (EPM). Rats' endocrine conditions were: a) Low hormone levels (rats in diestrus I and II phases); b) High hormone levels (proestrus/estrus phases); c) No hormones (ovariectomized rats); and d) Rats under progesterone withdrawal (PW). To evaluate the participation of the GABA_A receptor in the anxiolytic-like action of MT the antagonist picrotoxin was used.

Results: Results showed that MT induced dose-dependent anxiolytic-like actions in rats with low hormone level conditions. Also, MT reduced anxiety-like behavior in female rats under PW, in contrast to diazepam which was ineffective. MT's anxiolytic-like effect was blocked by picrotoxin, suggesting the participation of the GABA_A receptor complex. However, increased anxiety-like behavior was observed in rats with a high hormone level condition and low doses of MT.

Conclusions: Beneficial anxiolytic-like actions of MT are observed under low hormone conditions, particularly in the PW challenge (a condition that can be related to a premenstrual period). Furthermore, the participation of the GABA_A receptor is evidenced. However, hormonal variations could induce the opposite effects, hence women should be cautious.

Keywords: Anxiolytic-like effect; Elevated plus-maze; Estrous cycle; GABA(A) receptor; Montanoa tomentosa; Progesterone withdrawal.