CRISPR/Cas Multiplexed Biosensing: A Challenge or an Insurmountable Obstacle?

Yi Li; Linyang Liu; Guozhen Liu

doi:10.1016/j.tibtech.2019.04.012

CRISPR/Cas Multiplexed Biosensing: A Challenge or an Insurmountable Obstacle?

Trends Biotechnol. 2019 Aug;37(8):792-795. doi: 10.1016/j.tibtech.2019.04.012. Epub 2019 May 29.

Authors

Yi Li¹, Linyang Liu¹, Guozhen Liu²

Affiliations

¹ Graduate School of Biomedical Engineering, ARC Centre of Excellence in Nanoscale Biophotonics, Faculty of Engineering, University of New South Wales, Sydney, NSW 2052, Australia; Australian Centre for NanoMedicine, University of New South Wales, Sydney, NSW 2052, Australia.
² Graduate School of Biomedical Engineering, ARC Centre of Excellence in Nanoscale Biophotonics, Faculty of Engineering, University of New South Wales, Sydney, NSW 2052, Australia; Australian Centre for NanoMedicine, University of New South Wales, Sydney, NSW 2052, Australia; International Joint Research Center for Intelligent Biosensor Technology and Health, College of Chemistry, Central China Normal University, Wuhan 430079, PR China. Electronic address: guozhen.liu@unsw.edu.au.

PMID: 31153632
DOI: 10.1016/j.tibtech.2019.04.012

Abstract

Performing multiplex detection is still an elusive goal for molecular diagnostics. CRISPR/Cas-based biosensing has demonstrated potential for multiplex detection. Instead of being an insurmountable obstacle, CRISPR/Cas multiplexed biosensing is a realistic challenge with some recent successful applications. Strategic considerations are required to fully explore its potential in multiplex diagnostics.

Keywords: CRISPR/Cas; biosensing; diagnostics; multiplex; nucleic acid detection.

Publication types

Letter

MeSH terms

Biosensing Techniques / methods*
Biosensing Techniques / trends
CRISPR-Cas Systems*
Diagnostic Tests, Routine / methods*
Diagnostic Tests, Routine / trends
Humans
Molecular Diagnostic Techniques / methods*
Molecular Diagnostic Techniques / trends