Study of arsenic (III) removal by monolayer protected silver nanoadsorbent and its execution on prokaryotic system

Abstract

This work deals with the removal of arsenic by nanoadsorbent from aqueous environment that is subsequently applied to the biological system for the evaluation of its efficiency. We started our aspiration by the modification of carboxylate functionalized silver nanoparticle (nanoadsorbent) fabrication process. Batch mode arsenic uptake study by the nanoadsorbent was conducted considering several altering parameters and the reactants in addition to products were evaluated by several analytical techniques for the interpretation of the interaction mechanism. It was found nanoadsorbent, Ag@MSA is an efficient system for the exclusion of arsenic (III) from the aqueous system and due to the alteration in the ratio of silver content and protective agent, the characteristic profile of silver nanoparticles with an average diameter of 15 nm also became changed in respect of reported results. Here the low pH range (4-6) favors the interaction between nanoparticle and arsenic and it was found that the interaction was chemical in nature through adsorption or complex formation with surface carboxylate groups of the protecting agent (MSA). Following the interaction, a successful removal of arsenic (III) was achieved at a percentage of 94.16 with an initial concentration of 45 mg/L and equilibrium time of 60 min. Hence nanoparticles were executed against the toxic effect of arsenic in E. coli, an important gut microbe of higher animals, for the restoration of bacterial growth in arsenic pre-removed media. In this context the validation of this removal efficiency against arsenic induced toxicity was proved through several morphological studies, degree of oxidative damages and other biochemical attributes.

Keywords: Arsenic (III); Carboxylate functionalized; E.coli; Nanoadsorbent; Toxicity.