Persistence of intrathecal oligoclonal B cells and IgG in multiple sclerosis

Alina Tomescu-Baciu; Jorunn N Johansen; Trygve Holmøy; Victor Greiff; Maria Stensland; Gustavo Antonio de Souza; Frode Vartdal; Andreas Lossius

doi:10.1016/j.jneuroim.2019.576966

Persistence of intrathecal oligoclonal B cells and IgG in multiple sclerosis

J Neuroimmunol. 2019 Aug 15:333:576966. doi: 10.1016/j.jneuroim.2019.576966. Epub 2019 May 16.

Authors

Alina Tomescu-Baciu¹, Jorunn N Johansen¹, Trygve Holmøy², Victor Greiff³, Maria Stensland⁴, Gustavo Antonio de Souza⁴, Frode Vartdal¹, Andreas Lossius⁵

Affiliations

¹ Department of Immunology and Transfusion Medicine, Faculty of Medicine, University of Oslo and Oslo University Hospital Rikshospitalet, Oslo, Norway.
² Department of Neurology, Akershus University Hospital, Lørenskog, Norway; Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway.
³ Department of Immunology and Transfusion Medicine, Faculty of Medicine, University of Oslo and Oslo University Hospital Rikshospitalet, Oslo, Norway; Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway.
⁴ Department of Immunology and Transfusion Medicine, Faculty of Medicine, University of Oslo and Oslo University Hospital Rikshospitalet, Oslo, Norway; Proteomics Core Facility, Oslo University Hospital Rikshospitalet, NO-0372 Oslo, Norway.
⁵ Department of Immunology and Transfusion Medicine, Faculty of Medicine, University of Oslo and Oslo University Hospital Rikshospitalet, Oslo, Norway; Department of Neurology, Akershus University Hospital, Lørenskog, Norway. Electronic address: andreas.lossius@medisin.uio.no.

PMID: 31153015
DOI: 10.1016/j.jneuroim.2019.576966

Abstract

In multiple sclerosis (MS), B cells are trafficking across the blood-brain barrier, but it is not known how this relates to the synthesis of oligoclonal IgG. We used quantitative mass spectrometry of oligoclonal bands and high-throughput sequencing of immunoglobulin heavy-chain variable transcripts to study the longitudinal B cell response in the cerebrospinal fluid (CSF) and blood of two MS patients. Twenty of 22 (91%) and 25 of 28 (89%) of oligoclonal band peptides persisted in samples collected 18 months apart, in spite of a dynamic exchange across the blood-CSF barrier of B lineage cells connecting to oligoclonal IgG.

Keywords: B cells; Cerebrospinal fluid; Multiple sclerosis; Oligoclonal bands.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Amino Acid Sequence
B-Lymphocyte Subsets / immunology*
Blood-Brain Barrier
Cell Lineage
Female
Follow-Up Studies
Gene Rearrangement, B-Lymphocyte
High-Throughput Nucleotide Sequencing
Humans
Immunoglobulin G / biosynthesis
Immunoglobulin G / cerebrospinal fluid*
Immunoglobulin G / genetics
Immunoglobulin G / immunology
Immunoglobulin Variable Region / genetics
Immunologic Factors / therapeutic use
Multiple Sclerosis, Relapsing-Remitting / cerebrospinal fluid*
Multiple Sclerosis, Relapsing-Remitting / drug therapy
Multiple Sclerosis, Relapsing-Remitting / immunology
Oligoclonal Bands / cerebrospinal fluid*
Sequence Alignment
Sequence Homology, Amino Acid
Transcriptome
Young Adult

Substances

Immunoglobulin G
Immunoglobulin Variable Region
Immunologic Factors
Oligoclonal Bands