Colonic Delivery of H2S Donors for Studying Cardiovascular Effects of H2S in Rats

Marcin Ufnal; Tomasz Hutsch

doi:10.1007/978-1-4939-9528-8_13

Colonic Delivery of H₂S Donors for Studying Cardiovascular Effects of H₂S in Rats

Methods Mol Biol. 2019:2007:173-177. doi: 10.1007/978-1-4939-9528-8_13.

Authors

Marcin Ufnal¹, Tomasz Hutsch²

Affiliations

¹ Laboratory of Centre for Preclinical Research, Department of Experimental Physiology and Pathophysiology, Medical University of Warsaw, Warsaw, Poland. mufnal@wum.edu.pl.
² Laboratory of Centre for Preclinical Research, Department of Experimental Physiology and Pathophysiology, Medical University of Warsaw, Warsaw, Poland.

PMID: 31148114
DOI: 10.1007/978-1-4939-9528-8_13

Abstract

There is evidence that H₂S produced in the colon may contribute to the control of the circulatory system. This chapter describes a technique of cardiovascular measurements in anesthetized rats subjected to intracolonic administration of H₂S donor. The intracolonic administration is performed via polyurethane catheter inserted per rectum into the colon.

Keywords: Bacteria; Cardiovascular diseases; Colon; Gut; Hydrogen sulfide; i.c..

MeSH terms

Animals
Catheterization / methods*
Colon / metabolism*
Hemodynamics / drug effects*
Hydrogen Sulfide / metabolism
Hydrogen Sulfide / pharmacology*
Male
Rats

Substances

Hydrogen Sulfide